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The use of osmicated tissues for Lowicryl K4M embedding

A Nanci, M Mazariegos and M Fortin

Faculty of Dentistry, Universite de Montreal, Quebec, Canada.

Tissue chopper slices of rat incisor, rat parotid gland, and chicken tibiae, fixed with 1% glutaraldehyde, were post-fixed with potassium ferrocyanide-reduced osmium tetroxide, dehydrated with methanol, and conventionally embedded in Lowicryl K4M at -20 degrees C. The tissues showed an ultrastructural appearance comparable with that of their Epon- embedded counterparts and, in particular, the Golgi apparatus was well defined. Furthermore, Lowicryl K4M-embedded osmicated tissues permitted both post-embedding lectin-gold cytochemistry and immunogold labeling.

Volume 40, Issue 6, pp. 869-874, 06/01/1992
Copyright © 1992 by The Histochemical Society


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