|
|
|
|
 |
|||
|
|||
Techniques to optimize post-embedding single and double staining for amino acid neurotransmitters
KD Phend, RJ Weinberg and A Rustioni
Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill 27599.
We report a number of technical refinements for single and double staining with post-embedding electron microscopy for glutamate, aspartate, and gamma-aminobutyric acid. Best results were obtained with 2.5% glutaraldehyde in the fixative and by minimizing the duration of plastic polymerization and the interval between cutting and reacting. Quantitative documentation of the ability of exogenous glutamate, aspartate, and gamma-aminobutyric acid to block their immune staining is provided. Increased intensity of staining with the glutamate and aspartate antisera resulted from preincubation of glutamate antiserum with aspartate and aspartate antiserum with glutamate. To perform double staining with antisera raised in the same species, it was necessary to block antigenicity of the first antiserum; best results were obtained with hot paraformaldehyde fumes. By using a detergent instead of etching, these methods permitted the simultaneous visualization of tracers to identify neuroanatomic pathways.
Volume 40,
Issue 7,
pp. 1011-1020,
07/01/1992
Copyright © 1992 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J.-M. Fritschy, P. Panzanelli, J. E. Kralic, K. E. Vogt, and M. Sassoe-Pognetto Differential dependence of axo-dendritic and axo-somatic GABAergic synapses on GABAA receptors containing the alpha1 subunit in Purkinje cells. J. Neurosci., March 22, 2006; 26(12): 3245 - 3255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. S. Bamford, S. Robinson, R. D. Palmiter, J. A. Joyce, C. Moore, and C. K. Meshul Dopamine Modulates Release from Corticostriatal Terminals J. Neurosci., October 27, 2004; 24(43): 9541 - 9552. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Erisir and J. L. Harris Decline of the Critical Period of Visual Plasticity Is Concurrent with the Reduction of NR2B Subunit of the Synaptic NMDA Receptor in Layer 4 J. Neurosci., June 15, 2003; 23(12): 5208 - 5218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Ma, Y. Z. Huang, G. M. Pitcher, J. G. Valtschanoff, Y. H. Ma, L. Y. Feng, B. Lu, W. C. Xiong, M. W. Salter, R. J. Weinberg, et al. Ligand-Dependent Recruitment of the ErbB4 Signaling Complex into Neuronal Lipid Rafts J. Neurosci., April 15, 2003; 23(8): 3164 - 3175. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Satzler, L. F. Sohl, J. H. Bollmann, J. G. G. Borst, M. Frotscher, B. Sakmann, and J. H. R. Lubke Three-Dimensional Reconstruction of a Calyx of Held and Its Postsynaptic Principal Neuron in the Medial Nucleus of the Trapezoid Body J. Neurosci., December 15, 2002; 22(24): 10567 - 10579. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Burette, U. Zabel, R. J. Weinberg, H. H. H. W. Schmidt, and J. G. Valtschanoff Synaptic Localization of Nitric Oxide Synthase and Soluble Guanylyl Cyclase in the Hippocampus J. Neurosci., October 15, 2002; 22(20): 8961 - 8970. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. B. Levy and C. Aoki alpha 7 Nicotinic Acetylcholine Receptors Occur at Postsynaptic Densities of AMPA Receptor-Positive and -Negative Excitatory Synapses in Rat Sensory Cortex J. Neurosci., June 15, 2002; 22(12): 5001 - 5015. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Shostak, Y. Ding, J. Mavity-Hudson, and V. A. Casagrande Cortical Synaptic Arrangements of the Third Visual Pathway in Three Primate Species: Macaca mulatta, Saimiri sciureus, and Aotus trivirgatus J. Neurosci., April 1, 2002; 22(7): 2885 - 2893. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Rubinstein, C. Cepeda, R. S. Hurst, J. Flores-Hernandez, M. A. Ariano, T. L. Falzone, L. B. Kozell, C. K. Meshul, J. R. Bunzow, M. J. Low, et al. Dopamine D4 Receptor-Deficient Mice Display Cortical Hyperexcitability J. Neurosci., June 1, 2001; 21(11): 3756 - 3763. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. Valtschanoff and R. J. Weinberg Laminar Organization of the NMDA Receptor Complex within the Postsynaptic Density J. Neurosci., February 15, 2001; 21(4): 1211 - 1217. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Naisbitt, J. Valtschanoff, D. W. Allison, C. Sala, E. Kim, A. M. Craig, R. J. Weinberg, and M. Sheng Interaction of the Postsynaptic Density-95/Guanylate Kinase Domain-Associated Protein Complex with a Light Chain of Myosin-V and Dynein J. Neurosci., June 15, 2000; 20(12): 4524 - 4534. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kubota and Y. Kawaguchi Dependence of GABAergic Synaptic Areas on the Interneuron Type and Target Size J. Neurosci., January 1, 2000; 20(1): 375 - 386. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Zamanillo, R. Sprengel, O. Hvalby, V. Jensen, N. Burnashev, A. Rozov, K. M. Kaiser, H. J. Köster, T. Borchardt, P. Worley, et al. Importance of AMPA Receptors for Hippocampal Synaptic Plasticity But Not for Spatial Learning Science, June 11, 1999; 284(5421): 1805 - 1811. [Abstract] [Full Text]
|
|
|
|
|
|
V. Gundersen, F. A. Chaudhry, J. G. Bjaalie, F. Fonnum, O. P. Ottersen, and J. Storm-Mathisen Synaptic Vesicular Localization and Exocytosis of L-Aspartate in Excitatory Nerve Terminals: A Quantitative Immunogold Analysis in Rat Hippocampus J. Neurosci., August 15, 1998; 18(16): 6059 - 6070. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-P. Hsueh, F.-C. Yang, V. Kharazia, S. Naisbitt, A. R. Cohen, R. J. Weinberg, and M. Sheng Direct Interaction of CASK/LIN-2 and Syndecan Heparan Sulfate Proteoglycan and Their Overlapping Distribution in Neuronal Synapses J. Cell Biol., July 13, 1998; 142(1): 139 - 151. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Ingham, S. H. Hood, P. Taggart, and G. W. Arbuthnott Plasticity of Synapses in the Rat Neostriatum after Unilateral Lesion of the Nigrostriatal Dopaminergic Pathway J. Neurosci., June 15, 1998; 18(12): 4732 - 4743. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Pinault, Y. Smith, and M. Deschenes Dendrodendritic and Axoaxonic Synapses in the Thalamic Reticular Nucleus of the Adult Rat J. Neurosci., May 1, 1997; 17(9): 3215 - 3233. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Bevan, N. P. Clarke, and J. P. Bolam Synaptic Integration of Functionally Diverse Pallidal Information in the Entopeduncular Nucleus and Subthalamic Nucleus in the Rat J. Neurosci., January 1, 1997; 17(1): 308 - 324. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. W. Godwin, S. C. Van Horn, M. Sesma, C. Romano, and S. M. Sherman Ultrastructural Localization Suggests that Retinal and Cortical Inputs Access Different Metabotropic Glutamate Receptors in the Lateral Geniculate Nucleus J. Neurosci., December 15, 1996; 16(24): 8181 - 8192. [Abstract] [Full Text] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |