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p53 overexpression in formalin-fixed, paraffin-embedded tissue detected by immunohistochemistry
BJ Kerns, PA Jordan, MB Moore, PA Humphrey, A Berchuck, MF Kohler, RC Bast , JD Iglehart and JR Marks
Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710.
Mutation and overexpression of the p53 gene have been noted in a wide range of human cancers and are thought to play a role in malignant transformation. Previously, immunohistochemical detection of p53 has been possible only in fresh-frozen tissues. We examined p53 expression in paraffin-embedded tissues from 50 epithelial ovarian cancers and 25 primary breast cancers with a modified immunohistochemical (IHC) technique developed in this laboratory, using monoclonal antibody (MAb) PAb1801. The 75 cases were selected from a group of patients in whom the expression levels had already been assessed in a fresh-tissue IHC assay. An identical staining reactivity was observed in both formalin- fixed, paraffin-embedded tissue and fresh-frozen tissue in 48 of 50 (96%) epithelial ovarian cancers and in 23 of 25 (92%) primary breast cancers. Immunodetection of p53 in paraffin-embedded tissue blocks will be a useful alternative to the standard fresh-tissue assay and can accurately reflect the level of p53 expression in human tumors.
Volume 40,
Issue 7,
pp. 1047-1051,
07/01/1992
Copyright © 1992 by The Histochemical Society
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