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A simple enhancement method for the silver-gold-intensified diaminobenzidine reaction in the light microscopic immunoperoxidase technique

S Goto, S Nagahiro, Y Ushio and W Hofer

Department of Neurosurgery, Kumamoto University Medical School, Japan.

We describe a simple and sensitive method for enhancement of the silver- gold-intensified 3,3'-diaminobenzidine (DAB) reaction demonstrating peroxidase activity. After completing silver-gold intensification of the preparations immunostained by the avidin-biotin-peroxidase method with DAB as the chromogen, the preparations were immersed in a solution containing uranyl nitrate. This new method appeared to increase the sensitivity by at least one order of magnitude as compared with silver- gold intensification alone.

Volume 40, Issue 9, pp. 1423-1425, 09/01/1992
Copyright © 1992 by The Histochemical Society


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