|
|
|
|
 |
|||
|
|||
Differential expression of lectin-binding sites defines mouse intestinal M-cells
MA Clark, MA Jepson, NL Simmons, TA Booth and BH Hirst
Gastrointestinal Drug Delivery Research Centre, University of Newcastle upon Tyne, Medical School, United Kingdom.
We investigated the binding of four lectins to the follicle-associated epithelium (FAE) overlying fixed mouse small intestinal Peyer's patches to identify M-cell-specific surface markers. Wheat germ agglutinin and peanut agglutinin displayed heterogeneous staining patterns, binding most avidly to the intestine goblet cells. In contrast, the lectins Ulex europaeus 1 (UEA 1) and Psophocarpus tetragonolobus (winged bean; WBA) were almost exclusively M-cell specific. When confocal laser scanning images of tissues stained with fluorescein isothiocyanate (FITC)-conjugated UEA1 or WBA were compared with the appearance of the same tissues under the scanning electron microscope (SEM), UEA1 strongly stained 97.2% (106/109) of M-cells, 0.6% (3/516) enterocytes, and 0% (0/28) goblet cells, whereas WBA stained 100% (83/83) M-cells, 1.7% (6/361) enterocytes, and 5.3% (1/19) goblet cells. The M-cell specificity of the lectin binding was further demonstrated by localization of horseradish peroxidase (HRP)-conjugated lectins under the transmission electron microscope (TEM). This is the first demonstration of carbohydrates in the glycocalyx of M-cells that are not expressed elsewhere on the FAE surface. These carbohydrates not only provide a means to identify mouse M-cells by LM but may also contribute to the occurrence of specific interactions between microorganisms and the M-cell apical membrane.
Volume 41,
Issue 11,
pp. 1679-1687,
11/01/1993
Copyright © 1993 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Kanaya, K. Miyazawa, I. Takakura, W. Itani, K. Watanabe, S. Ohwada, H. Kitazawa, M. T. Rose, H. R. McConochie, H. Okano, et al. Differentiation of a murine intestinal epithelial cell line (MIE) toward the M cell lineage Am J Physiol Gastrointest Liver Physiol, August 1, 2008; 295(2): G273 - G284. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Terahara, M. Yoshida, O. Igarashi, T. Nochi, G. S. Pontes, K. Hase, H. Ohno, S. Kurokawa, M. Mejima, N. Takayama, et al. Comprehensive Gene Expression Profiling of Peyer's Patch M Cells, Villous M-Like Cells, and Intestinal Epithelial Cells J. Immunol., June 15, 2008; 180(12): 7840 - 7846. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Westphal, A. Lugering, J. von Wedel, C. von Eiff, C. Maaser, T. Spahn, G. Heusipp, M. A. Schmidt, H. Herbst, I. R. Williams, et al. Resistance of Chemokine Receptor 6-Deficient Mice to Yersinia Enterocolitica Infection: Evidence of Defective M-Cell Formation in Vivo Am. J. Pathol., March 1, 2008; 172(3): 671 - 680. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ohka, H. Igarashi, N. Nagata, M. Sakai, S. Koike, T. Nochi, H. Kiyono, and A. Nomoto Establishment of a Poliovirus Oral Infection System in Human Poliovirus Receptor-Expressing Transgenic Mice That Are Deficient in Alpha/Beta Interferon Receptor J. Virol., August 1, 2007; 81(15): 7902 - 7912. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Lugering, M. Floer, S. Westphal, C. Maaser, T. W. Spahn, M. A. Schmidt, W. Domschke, I. R. Williams, and T. Kucharzik Absence of CCR6 Inhibits CD4+ Regulatory T-Cell Development and M-Cell Formation inside Peyer's Patches Am. J. Pathol., June 1, 2005; 166(6): 1647 - 1654. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Castagliuolo, M. Sardina, P. Brun, C. DeRos, C. Mastrotto, L. Lovato, and G. Palu Clostridium difficile Toxin A Carboxyl-Terminus Peptide Lacking ADP-Ribosyltransferase Activity Acts as a Mucosal Adjuvant Infect. Immun., May 1, 2004; 72(5): 2827 - 2836. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Lo, W. Tynan, J. Dickerson, M. Scharf, J. Cooper, D. Byrne, D. Brayden, L. Higgins, C. Evans, and D. J. O'Mahony Cell culture modeling of specialized tissue: identification of genes expressed specifically by follicle-associated epithelium of Peyer's patch by expression profiling of Caco-2/Raji co-cultures Int. Immunol., January 1, 2004; 16(1): 91 - 99. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Gebert, I. Steinmetz, S. Fassbender, and K.-H. Wendlandt Antigen Transport into Peyer's Patches: Increased Uptake by Constant Numbers of M Cells Am. J. Pathol., January 1, 2004; 164(1): 65 - 72. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Ramirez and A. Gebert Vimentin-positive Cells in the Epithelium of Rabbit Ileal Villi Represent Cup Cells but not M-cells J. Histochem. Cytochem., November 1, 2003; 51(11): 1533 - 1544. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-S. Park, K. P. Francis, J. Yu, and P. P. Cleary Membranous Cells in Nasal-Associated Lymphoid Tissue: A Portal of Entry for the Respiratory Mucosal Pathogen Group A Streptococcus J. Immunol., September 1, 2003; 171(5): 2532 - 2537. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Helander, K. J. Silvey, N. J. Mantis, A. B. Hutchings, K. Chandran, W. T. Lucas, M. L. Nibert, and M. R. Neutra The Viral {sigma}1 Protein and Glycoconjugates Containing {alpha}2-3-Linked Sialic Acid Are Involved in Type 1 Reovirus Adherence to M Cell Apical Surfaces J. Virol., July 15, 2003; 77(14): 7964 - 7977. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Edelman, B. Westerlund-Wikstrom, S. Leskela, H. Kettunen, N. Rautonen, J. Apajalahti, and T. K. Korhonen In Vitro Adhesion Specificity of Indigenous Lactobacilli within the Avian Intestinal Tract Appl. Envir. Microbiol., October 1, 2002; 68(10): 5155 - 5159. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R J Fitzhenry, D J Pickard, E L Hartland, S Reece, G Dougan, A D Phillips, and G Frankel Intimin type influences the site of human intestinal mucosal colonisation by enterohaemorrhagic Escherichia coli O157:H7 Gut, February 1, 2002; 50(2): 180 - 185. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Lelouard, H. Reggio, C. Roy, A. Sahuquet, P. Mangeat, and P. Montcourrier Glycocalyx on Rabbit Intestinal M Cells Displays Carbohydrate Epitopes from Muc2 Infect. Immun., February 1, 2001; 69(2): 1061 - 1071. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Lelouard, A. Sahuquet, H. Reggio, and P. Montcourrier Rabbit M cells and dome enterocytes are distinct cell lineages J. Cell Sci., January 6, 2001; 114(11): 2077 - 2083. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C NICOLETTI Unsolved mysteries of intestinal M cells Gut, November 1, 2000; 47(5): 735 - 739. [Full Text] [PDF]
|
|
|
|
|
|
N. J. Mantis, A. Frey, and M. R. Neutra Accessibility of glycolipid and oligosaccharide epitopes on rabbit villus and follicle-associated epithelium Am J Physiol Gastrointest Liver Physiol, June 1, 2000; 278(6): G915 - G923. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. V. Golovkina, M. Shlomchik, L. Hannum, and A. Chervonsky Organogenic Role of B Lymphocytes in Mucosal Immunity Science, December 3, 1999; 286(5446): 1965 - 1968. [Abstract] [Full Text]
|
|
|
|
|
|
A. Gebert, S. Fassbender, K. Werner, and A. Weissferdt The Development of M Cells in Peyer's Patches Is Restricted to Specialized Dome-Associated Crypts Am. J. Pathol., May 1, 1999; 154(5): 1573 - 1582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Giannasca, K. T. Giannasca, A. M. Leichtner, and M. R. Neutra Human Intestinal M Cells Display the Sialyl Lewis A Antigen Infect. Immun., February 1, 1999; 67(2): 946 - 953. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Lelouard, H. Reggio, P. Mangeat, M. Neutra, and P. Montcourrier Mucin-Related Epitopes Distinguish M Cells and Enterocytes in Rabbit Appendix and Peyer's Patches Infect. Immun., January 1, 1999; 67(1): 357 - 367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. B. Jensen, J. T. Harty, and B. D. Jones Interactions of the Invasive Pathogens Salmonella typhimurium, Listeria monocytogenes, and Shigella flexneri with M Cells and Murine Peyer's Patches Infect. Immun., August 1, 1998; 66(8): 3758 - 3766. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Neutra V. Role of M cells in transepithelial transport of antigens and pathogens to the mucosal immune system Am J Physiol Gastrointest Liver Physiol, May 1, 1998; 274(5): G785 - G791. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Clark, B. H. Hirst, and M. A. Jepson M-Cell Surface beta 1 Integrin Expression and Invasin-Mediated Targeting of Yersinia pseudotuberculosis to Mouse Peyer's Patch M Cells Infect. Immun., March 1, 1998; 66(3): 1237 - 1243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Clark, B. H. Hirst, and M. A. Jepson Inoculum Composition and Salmonella Pathogenicity Island 1 Regulate M-Cell Invasion and Epithelial Destruction by Salmonella typhimurium Infect. Immun., February 1, 1998; 66(2): 724 - 731. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Gebert and W. Posselt Glycoconjugate Expression Defines the Origin and Differentiation Pathway of Intestinal M-cells J. Histochem. Cytochem., October 1, 1997; 45(10): 1341 - 1350. [Abstract] [Full Text] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |