|
|
|
|
 |
|||
|
|||
Quantitative immunohistochemical evaluation by image analysis of metallothionein in copper-loaded rat kidney
KW Schmid, JM Morgan, D Ofner, A Hittmair, S Haywood and B Jasani
Department of Pathology, University of Munster, Germany.
Kidneys of copper-loaded rats were investigated immunohistochemically with a directly peroxidase-conjugated monoclonal antibody against metallothionein (MT). By means of an image analyzing system the area and the staining intensity of MT immunoreactivity in the proximal convoluted tubule (PCT) cells were determined. In the present study we compared the data obtained by image analysis with analytically determined tissue copper and MT concentrations of rats fed a high- copper diet (1 g/kg) for 16 weeks and sacrificed sequentially during this period. Our results provide evidence that the area of MT immunoreactivity correlates significantly with tissue copper and MT concentrations. Both the copper and MT concentrations in kidney rose to a maximum at 8 weeks and remained constant thereafter. The observed rise in the staining intensity of MT in PCT cells to a maximum at 6 weeks, which subsequently declined, suggests a continuing redistribution of copper and MT in the kidneys even after a maximum of concentration copper and MT is reached in the tissue.
Volume 41,
Issue 5,
pp. 727-731,
05/01/1993
Copyright © 1993 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Saito, M. Kurasaki, T. Hosokawa, M. Okabe, T. Saito, Y. Fujii, K. Nagashima, and K. Yoshida Localization of the Induced Metallothionein and DNA Damage in Rat Kidney After Gold Injection J. Histochem. Cytochem., September 1, 2002; 50(9): 1263 - 1271. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kurasaki, M. Okabe, S. Saito, and M. Suzuki-Kurasaki Copper metabolism in the kidney of rats administered copper and copper-metallothionein Am J Physiol Renal Physiol, April 1, 1998; 274(4): F783 - F790. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Suzuki-Kurasaki, M. Okabe, and M. Kurasaki Copper-metallothionein in the Kidney of Macular Mice: A Model for Menkes Disease J. Histochem. Cytochem., November 1, 1997; 45(11): 1493 - 1502. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. G. Steg, M. Ziol, O. Tahlil, C. Robert, P. Masson, D. Pruneau, P. Bruneval, and P. Belichard Reduction of Intimal Hyperplasia by Naroparcil, a 4-Methylumbelliferyl ß-D-Xyloside Analogue, After Arterial Injury in the Hypercholesterolemic Rabbit Circ. Res., November 1, 1995; 77(5): 919 - 926. [Abstract] [Full Text]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |