|
|
|
|
 |
|||
|
|||
A simple and reliable method for correlative light and electron microscopic studies
J DeFelipe and A Fairen
Instituto Cajal, Madrid, Spain.
We describe in detail a simple method for flat-embedding that can be subsequently used in correlative light and electron microscopic studies. The method can be applied to any material suitable for electron microscopy and is especially useful for study of the synaptology and ultrastructural characteristics of immunocytochemically or morphologically identified neurons or their processes. We present here an example to show how accurately one can delineate the fine details of a complex axonal arborization impregnated with the Golgi method in the mouse cerebral cortex. Golgi-impregnated sections to be studied at the electron microscopic level are osmicated, dehydrated, infiltrated with Araldite resin, flat-embedded, and identified cells or processes photographed. Serial semi-thin sections (1-2 microns thick) are then cut with an ultramicrotome, examined with the light microscope, and the elements rephotographed. Selected semi-thin sections are then resectioned on the ultramicrotome at 60-70 nm and examined electron microscopically. This method allows the systematic and accurate localization of stained cells and processes throughout the successive steps of the procedure.
Volume 41,
Issue 5,
pp. 769-772,
05/01/1993
Copyright © 1993 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  L. Alonso-Nanclares, J. Gonzalez-Soriano, J. R. Rodriguez, and J. DeFelipe Gender differences in human cortical synaptic density PNAS, September 23, 2008; 105(38): 14615 - 14619. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Alonso-Nanclares, R. Garbelli, R. G. Sola, J. Pastor, L. Tassi, R. Spreafico, and J. DeFelipe Microanatomy of the dysplastic neocortex from epileptic patients Brain, January 1, 2005; 128(1): 158 - 173. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. DeFelipe, J.I. Arellano, A. Merchan-Perez, M.C. Gonzalez-Albo, K. Walton, and R. Llinas Spaceflight Induces Changes in the Synaptic Circuitry of the Postnatal Developing Neocortex Cereb Cortex, August 1, 2002; 12(8): 883 - 891. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. I. Arellano, J. DeFelipe, and A. Munoz PSA-NCAM Immunoreactivity in Chandelier Cell Axon Terminals of the Human Temporal Cortex Cereb Cortex, June 1, 2002; 12(6): 617 - 624. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Persico, E. Mengual, R. Moessner, S. F. Hall, R. S. Revay, I. Sora, J. Arellano, J. DeFelipe, J. M. Gimenez-Amaya, M. Conciatori, et al. Barrel Pattern Formation Requires Serotonin Uptake by Thalamocortical Afferents, and Not Vesicular Monoamine Release J. Neurosci., September 1, 2001; 21(17): 6862 - 6873. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. DeFelipe, P. Marco, I. Busturia, and A. Merchan-Perez Estimation of the Number of Synapses in the Cerebral Cortex: Methodological Considerations Cereb Cortex, October 1, 1999; 9(7): 722 - 732. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. J. Sun, L. P. Tolbert, J. G. Hildebrand, and I. A. Meinertzhagen A Rapid Method for Combined Laser Scanning Confocal Microscopic and Electron Microscopic Visualization of Biocytin or Neurobiotin-labeled Neurons J. Histochem. Cytochem., February 1, 1998; 46(2): 263 - 274. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Frade, P Bovolenta, J. Martinez-Morales, A Arribas, J. Barbas, and A Rodriguez-Tebar Control of early cell death by BDNF in the chick retina Development, January 9, 1997; 124(17): 3313 - 3320. [Abstract] [PDF]
|
|
|
|
|
|
P. Bovolenta, J.-M. Frade, E. Marti, M.-A. Rodriguez-Pena, Y.-A. Barde, and A. Rodriguez-Tebar Neurotrophin-3 Antibodies Disrupt the Normal Development of the Chick Retina J. Neurosci., July 15, 1996; 16(14): 4402 - 4410. [Abstract] [Full Text] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |