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Secretion of chondroitin sulfate from embryonic epidermal cells in Xenopus laevis

S Nishikawa and F Sasaki

Department of Biology, Tsurumi University, School of Dental Medicine, Yokohama, Japan.

We examined proteoglycans (PGs) in amphibian epidermal cells by immunofluorescence microscopy. Immunoelectron microscopy with pre- and post-embedding methods, combined with HRP- or gold-conjugated secondary antibody, revealed ultrastructural localization of glycosaminoglycans (GAGs). Embryonic epidermis secretion granules in Xenopus laevis contained chondroitin 6-sulfate and unsulfated chondroitin. Immature secretion granules were also labeled with anti-chondroitin 6-sulfate and anti-chondroitin. A step-wise digestion experiment on chondroitinase ABC revealed that fine filaments in the secretion granules were chondroitin sulfate chains. Inhibition experiments with brefeldin A revealed that the life of a secretion granule was 5-10 hr, suggesting that GAG secretion from embryonic epidermis is routed through a regulated pathway.

Volume 41, Issue 9, pp. 1373-1381, 09/01/1993
Copyright © 1993 by The Histochemical Society


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