Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Evers, P.
Right arrow Articles by Uylings, H. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Evers, P.
Right arrow Articles by Uylings, H. B.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Microwave-stimulated antigen retrieval is pH and temperature dependent

P Evers and HB Uylings

Netherlands Institute for Brain Research, Graduate School Neurosciences Amsterdam, Amsterdam.

The effects of the pH of solutions used in microwave-stimulated antigen retrieval were investigated for two antibodies commonly used in neurosciences, MAP-2 and SMI-32. A prolonged fixation time (longer than 1 week) prevents good immunohistochemical staining of these and many other antigens in paraffin and vibratome sections. On human brain tissue stored for 17 months in formaldehyde, the effects of different solutions during microwave treatment were compared, i.e., 4% aluminum chloride (pH 2.75), 0.1 N hydrochloric acid (pH 0.98), and citrate buffers (pH 2.5, 4.5, 6.0), together with the influence of temperature and microwave irradiation. We conclude that the pH and temperature of the solutions and the irradiation time are of great importance for the retrieval of antigens, but in a different way for the two antibodies studied. The SMI-32 staining results are optimal with citrate buffer at pH 2.5 and 2-hr irradiation at 90 degrees C, whereas MAP-2 staining results are optimal with citrate buffer at pH 4.5 and 10-min full-power irradiation. In the other conditions the staining is less intensive or less homogeneously distributed over the section.

Volume 42, Issue 12, pp. 1555-1563, 12/01/1994
Copyright © 1994 by The Histochemical Society


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Histochem. Cytochem.Home page
S. Namimatsu, M. Ghazizadeh, and Y. Sugisaki
Reversing the Effects of Formalin Fixation with Citraconic Anhydride and Heat: A Universal Antigen Retrieval Method
J. Histochem. Cytochem., January 1, 2005; 53(1): 3 - 11.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
N. Ohno, N. Terada, S.-i. Murata, R. Katoh, and S. Ohno
Application of Cryotechniques with Freeze-substitution for the Immunohistochemical Demonstration of Intranuclear pCREB and Chromosome Territory
J. Histochem. Cytochem., January 1, 2005; 53(1): 55 - 62.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
E. Zudaire, S. J. Simpson, I. Illa, and L. M. Montuenga
Dietary influences over proliferating cell nuclear antigen expression in the locust midgut
J. Exp. Biol., June 1, 2004; 207(13): 2255 - 2265.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
H. Ino
Antigen Retrieval by Heating En Bloc for Pre-fixed Frozen Material
J. Histochem. Cytochem., August 1, 2003; 51(8): 995 - 1003.
[Abstract] [Full Text] [PDF]

Home page
 Cereb CortexHome page
W. E. Kaufmann, S. M. MacDonald, and C. R. Altamura
Dendritic Cytoskeletal Protein Expression in Mental Retardation: An Immunohistochemical Study of the Neocortex in Rett Syndrome
Cereb Cortex, October 1, 2000; 10(10): 992 - 1004.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
G. Meyer, J. P. Schaaps, L. Moreau, and A. M. Goffinet
Embryonic and Early Fetal Development of the Human Neocortex
J. Neurosci., March 1, 2000; 20(5): 1858 - 1868.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
F. R. Fusco, Q. Chen, W. J. Lamoreaux, G. Figueredo-Cardenas, Y. Jiao, J. A. Coffman, D. J. Surmeier, M. G. Honig, L. R. Carlock, and A. Reiner
Cellular Localization of Huntingtin in Striatal and Cortical Neurons in Rats: Lack of Correlation with Neuronal Vulnerability in Huntington's Disease
J. Neurosci., February 15, 1999; 19(4): 1189 - 1202.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
F. Loup, O. Weinmann, Y. Yonekawa, A. Aguzzi, H.-G. Wieser, and J.-M. Fritschy
A Highly Sensitive Immunofluorescence Procedure for Analyzing the Subcellular Distribution of GABAA Receptor Subunits in the Human Brain
J. Histochem. Cytochem., October 1, 1998; 46(10): 1129 - 1140.
[Abstract] [Full Text]

Home page
 J. Histochem. Cytochem.Home page
C. M. Ferrier, W. L. van Geloof, H. H. de Witte, M. D. Kramer, D. J. Ruiter, and G. N.P. van Muijen
Epitopes of Components of the Plasminogen Activation System are Re-exposed in Formalin-fixed Paraffin Sections by Different Retrieval Techniques
J. Histochem. Cytochem., April 1, 1998; 46(4): 469 - 476.
[Abstract] [Full Text]

Home page
 J. Histochem. Cytochem.Home page
S.-R. Shi, R. J. Cote, and C. R. Taylor
Antigen Retrieval Immunohistochemistry: Past, Present, and Future
J. Histochem. Cytochem., March 1, 1997; 45(3): 327 - 344.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1994