|
|
|
|
 |
|||
|
|||
A protein-specific monoclonal antibody to rat liver beta 1-->4 galactosyltransferase and its application to immunohistochemistry
J Kawano, S Ide, T Oinuma and T Suganuma
Department of Anatomy, Miyazaki Medical College, Japan.
We have produced a new protein-specific monoclonal antibody (MAb) to rat liver beta 1-->4 galactosyltransferase. This MAb, GTL2, was selected as the most reactive IgG to a periodate-treated antigen. Antigen and protein specificities of GTL2 were verified by immunoblotting of a non-glycosylated recombinant protein of human galactosyltransferase and enzymatically deglycosylated rat galactosyltransferase. Using GTL2, an immunohistochemical study was done in rat liver, epididymis, and salivary glands. Intense staining was observed in Golgi areas of epididymal duct epithelial cells, and submandibular and sublingual acinar cells. Hepatocytes showed weaker staining. Immunoelectron microscopic observation revealed that the staining was exclusively localized in trans-Golgi membranes of these cells.
Volume 42,
Issue 3,
pp. 363-369,
03/01/1994
Copyright © 1994 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  H.-W. Shin, H. Kobayashi, M. Kitamura, S. Waguri, T. Suganuma, Y. Uchiyama, and K. Nakayama Roles of ARFRP1 (ADP-ribosylation factor-related protein 1) in post-Golgi membrane trafficking J. Cell Sci., September 1, 2005; 118(17): 4039 - 4048. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Harada, T. Kawaguchi, H. Kumemura, K. Terada, H. Ninomiya, E. Taniguchi, S. Hanada, S. Baba, M. Maeyama, H. Koga, et al. The Wilson Disease Protein ATP7B Resides in the Late Endosomes with Rab7 and the Niemann-Pick C1 Protein Am. J. Pathol., February 1, 2005; 166(2): 499 - 510. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Windheim and H.-G. Burgert Characterization of E3/49K, a Novel, Highly Glycosylated E3 Protein of the Epidemic Keratoconjunctivitis-Causing Adenovirus Type 19a J. Virol., January 15, 2002; 76(2): 755 - 766. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Seemann, E. J. Jokitalo, and G. Warren The Role of the Tethering Proteins p115 and GM130 in Transport through the Golgi Apparatus In Vivo Mol. Biol. Cell, February 1, 2000; 11(2): 635 - 645. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. Ichikawa, J. Nakayama, N. Sakura, T. Hashimoto, M. Fukuda, M. N. Fukuda, and T. Taki Expression of N-acetyllactosamine and {beta}1,4-Galactosyltransferase ({beta}4GalT-I) During Adenoma-Carcinoma Sequence in the Human Colorectum J. Histochem. Cytochem., December 1, 1999; 47(12): 1593 - 1602. [Abstract] [Full Text]
|
|
|
|
|
|
C. Valetti, D. M. Wetzel, M. Schrader, M. J. Hasbani, S. R. Gill, T. E. Kreis, and T. A. Schroer Role of Dynactin in Endocytic Traffic: Effects of Dynamitin Overexpression and Colocalization with CLIP-170 Mol. Biol. Cell, December 1, 1999; 10(12): 4107 - 4120. [Abstract] [Full Text]
|
|
|
|
|
|
J. Füllekrug, T. Suganuma, B. L. Tang, W. Hong, B. Storrie, and T. Nilsson Localization and Recycling of gp27 (hp24gamma 3): Complex Formation with Other p24 Family Members Mol. Biol. Cell, June 1, 1999; 10(6): 1939 - 1955. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. T. Shima, N. Cabrera-Poch, R. Pepperkok, and G. Warren An Ordered Inheritance Strategy for the Golgi Apparatus: Visualization of Mitotic Disassembly Reveals a Role for the Mitotic Spindle J. Cell Biol., May 18, 1998; 141(4): 955 - 966. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ponnambalam, M. Girotti, M. L. Yaspo, C. E. Owen, A. C. Perry, T. Suganuma, T. Nilsson, M. Fried, G. Banting, and G. Warren Primate homologues of rat TGN38: primary structure, expression and functional implications J. Cell Sci., March 1, 1996; 109(3): 675 - 685. [Abstract] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |