Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by McCarthy, K. J.
Right arrow Articles by Couchman, J. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by McCarthy, K. J.
Right arrow Articles by Couchman, J. R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Basement membrane-specific chondroitin sulfate proteoglycan is abnormally associated with the glomerular capillary basement membrane of diabetic rats

KJ McCarthy, DR Abrahamson, KR Bynum, PL St. John and JR Couchman

Department of Cell Biology, School of Medicine, University of Alabama at Birmingham 35294.

We have previously reported the production of monoclonal antibodies (MAb) recognizing the core protein of a basement membrane-specific chondroitin sulfate proteoglycan (BM-CSPG). Using immunohistochemical techniques, we have shown that BM-CSPG is present in almost every basement membrane, one exception being the normal glomerular capillary basement membrane (GBM), where it is absent. In the present study of mature kidneys we examined the distribution of BM-CSPG in streptozocin- induced diabetes mellitus in rats. We found BM-CSPG atypically associated with the GBM of diabetic animals as early as 1 month after induction of diabetes mellitus. Immunoelectron microscopy (IEM) of affected capillary loops showed BM-CSPG present in the subendothelial matrix in areas of GBM thickening and absent in areas where the GBM appears to be of normal thickness. Moreover, the association of BM-CSPG with regions of the pericapillary GBM affects the morphology of the capillary endothelial cells within these areas, directly displacing the cell body from the GBM proper and causing loss of fenestrae. These new data on BM-CSPG distribution reflect abnormal glomerular extracellular matrix protein biosynthesis/turnover in diabetes and suggest that BM- CSPG in the GBM might in turn affect normal capillary structure and/or function.

Volume 42, Issue 4, pp. 473-484, 04/01/1994
Copyright © 1994 by The Histochemical Society


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Nephrol Dial TransplantHome page
A. J. A. Groffen, J. H. Veerkamp, L. A. H. Monnens, and L. P. W. J. van den Heuvel
Recent insights into the structure and functions of heparan sulfate proteoglycans in the human glomerular basement membrane
Nephrol. Dial. Transplant., September 1, 1999; 14(9): 2119 - 2129.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Ghiselli, L. D. Siracusa, and R. V. Iozzo
Complete cDNA Cloning, Genomic Organization, Chromosomal Assignment, Functional Characterization of the Promoter, and Expression of the Murine Bamacan Gene
J. Biol. Chem., June 11, 1999; 274(24): 17384 - 17393.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
R.-R. Wu and J. R. Couchman
cDNA Cloning of the Basement Membrane Chondroitin Sulfate Proteoglycan Core Protein, Bamacan: A Five Domain Structure Including Coiled-Coil Motifs
J. Cell Biol., January 27, 1997; 136(2): 433 - 444.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. R. Couchman, R. Kapoor, M. Sthanam, and R.-R. Wu
Perlecan and Basement Membrane-Chondroitin Sulfate Proteoglycan (Bamacan) Are Two Basement Membrane Chondroitin/Dermatan Sulfate Proteoglycans in the Engelbreth-Holm-Swarm Tumor Matrix
J. Biol. Chem., April 19, 1996; 271(16): 9595 - 9602.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1994