Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Frenoy, J. P.
Right arrow Articles by Salmon, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Frenoy, J. P.
Right arrow Articles by Salmon, J. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Use of quantitative image microfluorometry to follow fluorescent ricin internalization in single living cells

JP Frenoy, F Emmanuel and JM Salmon

CNRS X 0036, Etats Lies Moleculaires, UFR Biomedicale des Saints-Peres, Universite Rene Descartes, Paris, France.

We used microspectrofluorometry and videomicrofluorometry to follow the binding and internalization of fluorescein-labeled toxic lectin ricin in living Zajdela hepatoma cells. Microspectrofluorometry showed that when ricin was specifically labeled on its B-chain with one molecule of fluorescein (ABF), its fluorescence spectrum did not alter during its binding to the cell surface and subsequent internalization. This enabled us to use image analysis to follow cell internalization of labeled ricin. Accordingly, we measured the appropriate fluorescent cell parameters, comprising total fluorescence intensity, cell surface area, mean fluorescence intensity and its standard error, and used the measurements for mono- and biparametric studies of cell fluorescence distribution. The results showed that (a) ricin binds two different subpopulations of Zajdela hepatoma cells, (b) Zajdela hepatoma cells internalize ricin rapidly and after a relatively stable period of 1-2 hr, internalization starts again at 4 hr, and (c) the distribution of intracellular fluorescence is heterogeneous and ABF accumulates in certain cellular localizations. Our results demonstrate that quantitative microfluorometry is an effective and interesting approach for real-time studies of macromolecule internalization in living cells.

Volume 42, Issue 5, pp. 627-633, 05/01/1994
Copyright © 1994 by The Histochemical Society


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1994