|
|
|
|
 |
|||
|
|||
Expression of mRNA for IL1 beta, IL6 and TGF beta 1 in developing human bone and cartilage
RA Dodds, K Merry, A Littlewood and M Gowen
Bath Institute for Rheumatic Diseases, United Kingdom.
Using in situ hybridization, we investigated the expression of mRNA for interleukin-1 beta (IL1 beta), interleukin-6 (IL6), and transforming growth factor-beta-1 (TGF beta 1) in sections of developing bone in human osteophytes. The expression was related to the cellular activity of alkaline phosphatase to aid in the identification of pre-osteoblast populations. IL1 beta mRNA was localized in active osteoblasts within distinct areas of intramembranous ossification. However, the expression was sporadic and appeared to occur at a specific stage of the osteoblast life cycle. There was no IL1 beta mRNA expression in any cell types during endochondral ossification. IL6 mRNA expression was located within pre-osteoblasts and in newly differentiated and matrix- secreting osteoblasts; expression was absent or reduced in flattened, inactive osteoblasts. Weak or no IL6 expression was observed in chondroblasts and chondrocytes, respectively. However, there was a close association between IL6 mRNA expression and the differentiation of mesenchymal cells into osteoblasts. TGF beta 1 expression was localized to osteoblasts apposed to bone or cartilage matrix; the intensity of expression correlated with matrix secretion. Chondroblasts and chondrocytes expressed lower but significant levels of TGF beta 1 mRNA; the expression was lost with the progression to calcifying cartilage. The three cytokines studied were differentially expressed both temporally and spatially, suggesting different roles for each in osteoblast and chondrocyte function.
Volume 42,
Issue 6,
pp. 733-744,
06/01/1994
Copyright © 1994 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  E. Pavel, K. Nadella, W. H. Towns II, and L. S. Kirschner Mutation of Prkar1a Causes Osteoblast Neoplasia Driven by Dysregulation of Protein Kinase A Mol. Endocrinol., February 1, 2008; 22(2): 430 - 440. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. G. Gunn, A. Conley, L. Deininger, S. D. Olson, D. J. Prockop, and C. A. Gregory A Crosstalk Between Myeloma Cells and Marrow Stromal Cells Stimulates Production of DKK1 and Interleukin-6: A Potential Role in the Development of Lytic Bone Disease and Tumor Progression in Multiple Myeloma Stem Cells, April 1, 2006; 24(4): 986 - 991. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Oshima, N. Watanabe, K.-i. Matsuda, S. Takai, M. Kawata, and T. Kubo Behavior of Transplanted Bone Marrow-derived GFP Mesenchymal Cells in Osteochondral Defect as a Simulation of Autologous Transplantation J. Histochem. Cytochem., February 1, 2005; 53(2): 207 - 216. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Scharstuhl, H. L. Glansbeek, H. M. van Beuningen, E. L. Vitters, P. M. van der Kraan, and W. B. van den Berg Inhibition of Endogenous TGF-{beta} During Experimental Osteoarthritis Prevents Osteophyte Formation and Impairs Cartilage Repair J. Immunol., July 1, 2002; 169(1): 507 - 514. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. B. Saadeh, B. J. Mehrara, D. S. Steinbrech, J. A. Spector, J. A. Greenwald, G. S. Chin, H. Ueno, G. K. Gittes, and M. T. Longaker Mechanisms of Fibroblast Growth Factor-2 Modulation of Vascular Endothelial Growth Factor Expression by Osteoblastic Cells Endocrinology, June 1, 2000; 141(6): 2075 - 2083. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. B. Saadeh, B. J. Mehrara, D. S. Steinbrech, M. E. Dudziak, J. A. Greenwald, J. S. Luchs, J. A. Spector, H. Ueno, G. K. Gittes, and M. T. Longaker Transforming growth factor-beta 1 modulates the expression of vascular endothelial growth factor by osteoblasts Am J Physiol Cell Physiol, October 1, 1999; 277(4): C628 - C637. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Agarwal, C. S. Chandra, N. P. Piesco, H. H. Langkamp, L. Bowen, and C. Baran Regulation of Periodontal Ligament Cell Functions by Interleukin-1beta Infect. Immun., March 1, 1998; 66(3): 932 - 937. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Sack, U. Burkhardt, M. Borte, H. Schädlich, K. Berg, and F. Emmrich Age-Dependent Levels of Select Immunological Mediators in Sera of Healthy Children Clin. Vaccine Immunol., January 1, 1998; 5(1): 28 - 32. [Abstract] [Full Text]
|
|
|
|
|
|
R. S. Taichman and S. G. Emerson The Role of Osteoblasts in the Hematopoietic Microenvironment Stem Cells, January 1, 1998; 16(1): 7 - 15. [Abstract] [Full Text]
|
|
|
|
|
|
C. K. Ritchie, L. R. Andrews, K. G. Thomas, D. J. Tindall, and L. A. Fitzpatrick The Effects of Growth Factors Associated with Osteoblasts on Prostate Carcinoma Proliferation and Chemotaxis: Implications for the Development of Metastatic Disease Endocrinology, March 1, 1997; 138(3): 1145 - 1150. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.C. Quintero, N.P. Piesco, H.H. Langkamp, L. Bowen, and S. Agarwal LPS Responsiveness in Periodontal Ligament Cells is Regulated by Tumor Necrosis Factor-{alpha} Journal of Dental Research, November 1, 1995; 74(11): 1802 - 1811. [Abstract] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |