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Estimation of S-phase fraction in tumor tissue sections by immunohistochemical staining of PCNA

K Sasaki, A Kurose, Y Ishida and M Matsuta

Department of Pathology, Iwate Medical University, Morioka-shi, Japan.

We describe a method for measuring the size of the S-phase fraction in human tumor tissue sections using an antibody to PCNA (PC10). Although treatment with Triton X-100 before fixation extracted a large amount of PCNA from the cells even in frozen tissue sections, PCNA remained exclusively in S-phase cells. Immunohistochemical staining of PCNA after the detergent treatment allowed estimation of the S-phase fraction in solid tumors. The validity of the method was directly proven by double staining of bromodeoxyuridine (BrdU) and PCNA in HeLa cells. The PCNA-positive cells were identical with BrdUrd-positive cells after the detergent treatment. In contrast, almost all HeLa cells in the exponentially growing phase were positive for PC10 without treatment with Triton X-100.

Volume 42, Issue 7, pp. 957-960, 07/01/1994
Copyright © 1994 by The Histochemical Society


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