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Use of double labeling and photo CD for morphometric analysis of injured skeletal muscle

WT Stauber, GR Miller, MJ Gibala and JD MacDougall

Department of Physiology, West Virginia University, Morgantown, USA.

We used computer-assisted analysis of myofiber cross-sectional areas to measure skeletal muscle responses to injury and disease. We developed a simple, inexpensive method for measuring myofiber size in human muscle samples using Kodak photo compact discs (CDs) as the image source. The photo CD serves as a permanent image storage medium and provides a high- resolution image that can be used to detect small myofibers. The use of double labeling for dystrophin and desmin allowed positive identification of both degenerating and regenerating fibers in a single biopsy specimen.

Volume 43, Issue 11, pp. 1179-1184, 11/01/1995
Copyright © 1995 by The Histochemical Society


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The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1995