|
|
|
|
 |
|||
|
|||
Immunocytochemical detection of phosphatidylinositol 4,5-bisphosphate localization sites within the nucleus
G Mazzotti, N Zini, E Rizzi, R Rizzoli, A Galanzi, A Ognibene, S Santi, A Matteucci, AM Martelli and NM Maraldi
Institute of Anatomia Umana Normale, University of Bologna, Italy.
Phosphatidylinositol 4,5-bisphosphate (PIP2) is a key element of signal transduction, being the preferential substrate of specific phospholipases that produce second messengers such as inositol trisphosphate (IP3) and diacylglycerol (DG). Because PIP2 has been cytochemically identified by monoclonal antibodies not only in the cytoplasmic membranes but also in the nuclear envelope and within the nucleus, we performed a study by immunoblotting and by confocal and electron microscopic immunocytochemistry to identify the nuclear sites of PIP2 localization and to exclude any cross-reactivity of the antibody with other nuclear molecules. The results confirm the specificity of the immunolabeling and indicate that PIP2 is localized at precise intranuclear sites both in in situ and in isolated nuclei. They also show that a significant amount of the phospholipid is retained by the cytoskeleton and by the inner nuclear matrix in in situ matrix preparations. Moreover the sensitivity of the immunocytochemical reaction is capable of detecting quantitative variations of PIP2 nuclear content induced by agonists that modulate the signal transduction system at the nuclear level.
Volume 43,
Issue 2,
pp. 181-191,
02/01/1995
Copyright © 1995 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Tamguney and D. Stokoe New insights into PTEN J. Cell Sci., December 1, 2007; 120(23): 4071 - 4079. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Szivak, N. Lamb, and L. M. G. Heilmeyer Subcellular Localization and Structural Function of Endogenous Phosphorylated Phosphatidylinositol 4-Kinase (PI4K92) J. Biol. Chem., June 16, 2006; 281(24): 16740 - 16749. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Avazeri, A.-M. Courtot, and B. Lefevre Regulation of spontaneous meiosis resumption in mouse oocytes by various conventional PKC isozymes depends on cellular compartmentalization J. Cell Sci., October 1, 2004; 117(21): 4969 - 4978. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. W. Ledeen and G. Wu Nuclear lipids: key signaling effectors in the nervous system and other tissues J. Lipid Res., January 1, 2004; 45(1): 1 - 8. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. F. Leite, E. C. Thrower, W. Echevarria, P. Koulen, K. Hirata, A. M. Bennett, B. E. Ehrlich, and M. H. Nathanson Nuclear and cytosolic calcium are regulated independently PNAS, March 4, 2003; 100(5): 2975 - 2980. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Varnai, X. Lin, S. B. Lee, G. Tuymetova, T. Bondeva, A. Spat, S. G. Rhee, G. Hajnoczky, and T. Balla Inositol Lipid Binding and Membrane Localization of Isolated Pleckstrin Homology (PH) Domains. STUDIES ON THE PH DOMAINS OF PHOSPHOLIPASE C delta 1 AND p130 J. Biol. Chem., July 19, 2002; 277(30): 27412 - 27422. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Osborne, C. L. Thomas, S. Gschmeissner, and G. Schiavo Nuclear PtdIns(4,5)P2 assembles in a mitotically regulated particle involved in pre-mRNA splicing J. Cell Sci., January 7, 2001; 114(13): 2501 - 2511. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. S. Gilmour and M. D. Mitchell Nuclear Lipid Signaling: Novel Role of Eicosanoids Experimental Biology and Medicine, January 1, 2001; 226(1): 1 - 4. [Abstract] [Full Text]
|
|
|
|
 |
|
 N. Avazeri, A.-M. Courtot, A. Pesty, C. Duquenne, and B. Lefèvre Cytoplasmic and Nuclear Phospholipase C-beta 1 Relocation: Role in Resumption of Meiosis in the Mouse Oocyte Mol. Biol. Cell, December 1, 2000; 11(12): 4369 - 4380. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. B. Lachyankar, N. Sultana, C. M. Schonhoff, P. Mitra, W. Poluha, S. Lambert, P. J. Quesenberry, N. S. Litofsky, L. D. Recht, R. Nabi, et al. A Role for Nuclear PTEN in Neuronal Differentiation J. Neurosci., February 15, 2000; 20(4): 1404 - 1413. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Oancea, M. N. Teruel, A. F.G. Quest, and T. Meyer Green Fluorescent Protein (GFP)-tagged Cysteine-rich Domains from Protein Kinase C as Fluorescent Indicators for Diacylglycerol Signaling in Living Cells J. Cell Biol., February 9, 1998; 140(3): 485 - 498. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Fricker, M. Hollinshead, N. White, and D. Vaux Interphase Nuclei of Many Mammalian Cell Types Contain Deep, Dynamic, Tubular Membrane-bound Invaginations of the Nuclear Envelope J. Cell Biol., February 10, 1997; 136(3): 531 - 544. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Bacqueville, P. Deleris, C. Mendre, M.-T. Pieraggi, H. Chap, G. Guillon, B. Perret, and M. Breton-Douillon Characterization of a G Protein-activated Phosphoinositide 3-Kinase in Vascular Smooth Muscle Cell Nuclei J. Biol. Chem., June 15, 2001; 276(25): 22170 - 22176. [Abstract] [Full Text] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |