|
|
|
|
 |
|||
|
|||
The septoclast, a cathepsin B-rich cell involved in the resorption of growth plate cartilage
ER Lee, L Lamplugh, NL Shepard and JS Mort
Electron Microscopy Unit, Shriners Hospital for Crippled Children, Montreal, Quebec, Canada.
At the transition between growth plate cartilage and the endochondral bone region, transverse septa are being eroded to allow the advance of invasive capillaries. To find out whether resorption is due to proteinase activity, tissue sections prepared from the growth plate/metaphyseal interface of young rats were immunostained with antibodies to the cysteine proteinase cathepsin B. Intense staining was found in a cell that is associated with the growing portion of the invasive capillaries and extends between them and the transverse septum. This cell has a single nucleus, actively synthesizes protein, and shows two other characteristic features: the cytoplasm is packed with multivesicular and dense bodies rich in cathepsin B, and the cell apex ends in a ruffled border extending into the transverse septum and signs of dissociated extracellular matrix. Even though the ruffled border resembles that of the osteoclast, the cell was not immunostained by a monoclonal antibody that recognizes a 97 KD protein known as ED1 which characterizes rat osteoclasts, monocytes, and macrophages. Therefore, this distinctive cell produces the proteinase cathepsin B and appears to be involved in the resorption of the transverse septum. The cell has been named the "septoclast."
Volume 43,
Issue 5,
pp. 525-536,
05/01/1995
Copyright © 1995 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  B. Ytrehus, C. S. Carlson, and S. Ekman Etiology and Pathogenesis of Osteochondrosis Vet. Pathol., July 1, 2007; 44(4): 429 - 448. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. R. Lee, L. Lamplugh, B. Kluczyk, J. S. Mort, and C. P. Leblond Protease Analysis by Neoepitope Approach Reveals the Activation of MMP-9 Is Achieved Proteolytically in a Test Tissue Cartilage Model Involved in Bone Formation J. Histochem. Cytochem., September 1, 2006; 54(9): 965 - 980. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Suzumoto, M. Takami, and T. Sasaki Differentiation and function of osteoclasts cultured on bone and cartilage J. Electron Microsc. (Tokyo), December 1, 2005; 54(6): 529 - 540. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. S. Adams and I. M. Shapiro THE FATE OF THE TERMINALLY DIFFERENTIATED CHONDROCYTE: EVIDENCE FOR MICROENVIRONMENTAL REGULATION OF CHONDROCYTE APOPTOSIS Critical Reviews in Oral Biology & Medicine, November 1, 2002; 13(6): 465 - 473. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C Faucheux, S Nesbitt, M Horton, and J Price Cells in regenerating deer antler cartilage provide a microenvironment that supports osteoclast differentiation J. Exp. Biol., January 2, 2001; 204(3): 443 - 455. [Abstract] [PDF]
|
|
|
|
 |
|
 R. G. Erben, S. Raith, J. Eberle, and M. Stangassinger Ovariectomy augments B lymphopoiesis and generation of monocyte-macrophage precursors in rat bone marrow Am J Physiol Endocrinol Metab, March 1, 1998; 274(3): E476 - E483. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. G. Erben Embedding of Bone Samples in Methylmethacrylate: An Improved Method Suitable for Bone Histomorphometry, Histochemistry, and Immunohistochemistry J. Histochem. Cytochem., February 1, 1997; 45(2): 307 - 314. [Abstract] [Full Text] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |
