|
|
|
|
 |
|||
|
|||
Differential subcellular localization of neural isoforms of the catalytic subunit of calmodulin-dependent protein phosphatase (calcineurin) in central nervous system neurons: immunohistochemistry on formalin-fixed paraffin sections employing antigen retrieval by microwave irradiation
N Usuda, H Arai, H Sasaki, T Hanai, T Nagata, T Muramatsu, RL Kincaid and S Higuchi
Department of Anatomy and Cell Biology, Shinshu University School of Medicine, Matsumoto, Japan.
We examined the immunohistochemical distribution of the two mammalian isoforms of calcineurin catalyic subunits, A alpha and A beta, in central nervous system (CNS) tissues of cows, rats, and humans. Cryostat sections and paraffin sections of parformaldehyde-fixed tissues were stained with antipeptide antibodies for each isoform. The same localization pattern was observed in both cryostat and paraffin sections. In the latter, the intensity of the staining was dramatically enhanced by microwave irradiation. Calcineurin isoforms were localized in a variety of nerve cells but not in neuroglial cells. Their differential expression as the A alpha isoform in the nucleus and the A beta isoform in the cytoplasm was present in a variety of CNS nerve cells, most distinctively in Purkinje cells of the cerebellum and pyramidal cells of the cerebrum, irrespective of species. These results suggest that each isform has distinct intracellular sites of action in CNS neurons and that the phenomenon has been conserved during mammalian evolution.
Volume 44,
Issue 1,
pp. 13-18,
01/01/1996
Copyright © 1996 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  R. I. Jabr, A. J. Wilson, M. H. Riddervold, A. H. Jenkins, B. A. Perrino, and L. H. Clapp Nuclear translocation of calcineurin Abeta but not calcineurin A{alpha} by platelet-derived growth factor in rat aortic smooth muscle Am J Physiol Cell Physiol, June 1, 2007; 292(6): C2213 - C2225. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Gooch An emerging role for calcineurin A{alpha} in the development and function of the kidney Am J Physiol Renal Physiol, April 1, 2006; 290(4): F769 - F776. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Sun, H. C. Blair, Y. Peng, N. Zaidi, O. A. Adebanjo, X. B. Wu, X. Y. Wu, J. Iqbal, S. Epstein, E. Abe, et al. Calcineurin regulates bone formation by the osteoblast PNAS, November 22, 2005; 102(47): 17130 - 17135. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Launey, S. Endo, R. Sakai, J. Harano, and M. Ito Protein phosphatase 2A inhibition induces cerebellar long-term depression and declustering of synaptic AMPA receptor PNAS, January 13, 2004; 101(2): 676 - 681. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Parsons, B. J. Wilkins, O. F. Bueno, and J. D. Molkentin Altered Skeletal Muscle Phenotypes in Calcineurin A{alpha} and A{beta} Gene-Targeted Mice Mol. Cell. Biol., June 15, 2003; 23(12): 4331 - 4343. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. N. Jovanovic, T. S. Sihra, A. C. Nairn, H. C. Hemmings Jr, P. Greengard, and A. J. Czernik Opposing Changes in Phosphorylation of Specific Sites in Synapsin I During Ca2+-Dependent Glutamate Release in Isolated Nerve Terminals J. Neurosci., October 15, 2001; 21(20): 7944 - 7953. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Nakazawa, N. Usuda, T. Matsui, T. Hanai, S. Matsushita, H. Arai, H. Sasaki, and S. Higuchi Localization of Calcineurin in the Mature and Developing Retina J. Histochem. Cytochem., February 1, 2001; 49(2): 187 - 196. [Abstract] [Full Text]
|
|
|
|
 |
|
 F. Rusnak and P. Mertz Calcineurin: Form and Function Physiol Rev, October 1, 2000; 80(4): 1483 - 1521. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Zhuo, W. Zhang, H. Son, I. Mansuy, R. A. Sobel, J. Seidman, and E. R. Kandel A selective role of calcineurin Aalpha in synaptic depotentiation in hippocampus PNAS, April 13, 1999; 96(8): 4650 - 4655. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-R. Shi, R. J. Cote, and C. R. Taylor Antigen Retrieval Immunohistochemistry: Past, Present, and Future J. Histochem. Cytochem., March 1, 1997; 45(3): 327 - 344. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-Y. Fan, J. Pan, R. Chu, D. Lee, K. D. Kluckman, N. Usuda, I. Singh, A. V. Yeldandi, M. S. Rao, N. Maeda, et al. Hepatocellular and Hepatic Peroxisomal Alterations in Mice with a Disrupted Peroxisomal Fatty Acyl-coenzyme A Oxidase Gene J. Biol. Chem., October 4, 1996; 271(40): 24698 - 24710. [Abstract] [Full Text] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |