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A differentiated silver intensification procedure for the peroxidase- diaminobenzidine reaction

B Quinn and AM Graybiel

Department of Brain & Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, USA.

Several silver-based procedures have been developed to intensify the peroxidase-catalyzed diaminobenzidine polymer used as a marker in immunohistochemistry. Many of these methods use acutely unstable reagents or reagents that are not amenable to batch processing. We describe a new procedure with an initial treatment in dilute ammoniacal silver, followed by a differentiation step to remove background staining and a final toning in 0.2% gold chloride. The procedure provides a jet-black intensification of the tan diaminobenzidine product, is amenable to batch processing of free-floating sections and slide-mounted sections, and does not require acid-cleaned glassware.

Volume 44, Issue 1, pp. 71-74, 01/01/1996
Copyright © 1996 by The Histochemical Society


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The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1996