|
|
|
|
 |
|||
|
|||
Localization of laminin-5 in the epidermal basement membrane
T Masunaga, H Shimizu, A Ishiko, Y Tomita, D Aberdam, JP Ortonne and T Nishikawa
Department of Dermatology, Keio University School of Medicine, Tokyo, Japan.
Laminin-5 (kalinin/nicein/BM600) is a component of the epidermal basement membrane zone. Previous en bloc pre-embedding immunogold electron microscopy (EM) has shown that the epitope of GB3, a monoclonal antibody against laminin-5, is present in the lamina lucida (LL). However, precise localization of the entire laminin-5 molecule was unclear because of uneven and limited penetration of gold-labeled antibody in pre-embedding immunolabeling. In addition, the location of the GB3 epitope may not directly represent the location of the laminin- 5 moelcule itself. To elucidate the precise ultrastructural distribution of the entire laminin-5 molecule, we used polyclonal antibodies against different sites of laminin-5. Dual staining immunofluorescence with anti-laminin-5 and anti-melanocyte antibodies and immunoperoxidase EM showed that laminin-5 was present only beneath keratinocytes and not beneath melanocytes. Both cryoultramicrotomy and postembedding immunogold EM demonstrated that laminin-5 was localized to the lamina densa (LD) and the lower LL, with major labeling beneath the hemidesmosome. Quantitative analysis showed that 67-69% of gold particles were distributed to the LD and 88-90% were distributed beneath the hemidesmosome. Our results indicate that laminin-5 is localized mainly to the LD and partially to the lower LL, and is associated predominantly with hemidesmosomes.
Volume 44,
Issue 11,
pp. 1223-1230,
11/01/1996
Copyright © 1996 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. R. McMillan, M. Akiyama, H. Nakamura, and H. Shimizu Colocalization of Multiple Laminin Isoforms Predominantly beneath Hemidesmosomes in the Upper Lamina Densa of the Epidermal Basement Membrane J. Histochem. Cytochem., January 1, 2006; 54(1): 109 - 118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. McMillan, M. Akiyama, and H. Shimizu Ultrastructural Orientation of Laminin 5 in the Epidermal Basement Membrane: an Updated Model for Basement Membrane Organization J. Histochem. Cytochem., October 1, 2003; 51(10): 1299 - 1306. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Gagnoux-Palacios, M. Allegra, F. Spirito, O. Pommeret, C. Romero, J.-p. Ortonne, and G. Meneguzzi The Short Arm of the Laminin {gamma}2 Chain Plays a Pivotal Role in the Incorporation of Laminin 5 into the Extracellular Matrix and in Cell Adhesion J. Cell Biol., May 14, 2001; 153(4): 835 - 850. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. F. Jonkman, J. Schuur, F. Dijk, K. Heeres, M. C. J. M. de Jong, J. B. van der Meer, K. B. Yancey, and H. H. Pas Inflammatory Variant of Epidermolysis Bullosa Acquisita With IgG Autoantibodies Against Type VII Collagen and Laminin {alpha}3 Arch Dermatol, February 1, 2000; 136(2): 227 - 231. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Goldfinger, S. Hopkinson, G. deHart, S Collawn, J. Couchman, and J. Jones The alpha3 laminin subunit, alpha6beta4 and alpha3beta1 integrin coordinately regulate wound healing in cultured epithelial cells and in the skin J. Cell Sci., January 8, 1999; 112(16): 2615 - 2629. [Abstract] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |