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Expression of the pituitary transcription factor GHF-1/PIT-1 in cell types of the adult porcine adenohypophysis

MM Malagon, JC Garrido, C Dieulois, C Hera, JL Castrillo, PM Dobado-Berrios and F Gracia-Navarro

Departmento de Biologia Celular, Facultad de Ciencias, Universidad de Cordoba, Spain.

We describe the expression of the transcription factor GHF-1/PIT-1 in adult porcine adenohypophysis by a nonradioactive in situ hybridization (ISH) method using a digoxigenin-labeled cDNA probe corresponding to the entire coding region of rat GHF-1. GHF-1 transcripts were found in 71.7% of adenohypophyseal cells. We also report the simultaneous detection of GHF-1 mRNA and pituitary hormones by combined ISH and immunocytochemistry (IC) in dispersed adenohypophyseal cells, detected with an alkaline phosphatase-NBT/BCIP technique and with an immunoperoxidase-3-amino-9-ethylcarbazole (AEC) method, respectively. The combination of the two techniques neither abolished nor diminished their sensitivity or specificity. GHF-1 is expressed in all five of the cell types in the adult porcine adenohypophysis, showing that this method is suitable for simultaneous detection of transcripts and proteins at the single-cell level.

Volume 44, Issue 6, pp. 621-627, 06/01/1996
Copyright © 1996 by The Histochemical Society


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J. L. Gonzalez de Aguilar, M. M. Malagon, R. M. Vazquez-Martinez, I. Lihrmann, M.-C. Tonon, H. Vaudry, and F. Gracia-Navarro
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