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Retrieval of leukocyte antigens in paraffin-embedded rat tissues

JD Shetye, A Scheynius, HT Mellstedt and P Biberfeld

Department of Oncology-Pathology, Karolinska Institute and Hospital, Stockholm, Sweden.

We compared the immunohistochemical reactivity of various rat leukocyte antigens in frozen and paraffin-embedded thymus, spleen, abdominal lymph node, liver, and brain tissues of healthy Sprague-Dawley rats, fixed in various fixatives. Immune reactivity after fixation in Methacarn was superior to that of 4% neutral buffered formalin, a mercury-based fixative (B-5), or Carnoy. Microwave (MW) antigen retrieval (AR) enhanced antigen reactivity. Ten of the 11 leukocyte antigens studied could be retrieved in Methacarn-fixed, paraffin- embedded sections with a reactivity comparable to that obtained on frozen sections.

Volume 44, Issue 7, pp. 767-776, 07/01/1996
Copyright © 1996 by The Histochemical Society


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