|
|
|
|
 |
|||
|
|||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
M-cells in the rabbit tonsil exhibit distinctive glycoconjugates in their apical membranes
A Gebert
Center of Anatomy, Medical School of Hannover, Germany.
The tonsil crypt epithelium contains membranous (M)-cells that transport antigens from the lumen to underlying lymphoid cells, thereby initiating specific immune responses. Mechanisms mediating the adhesion of antigens to the M-cell surface are important for effective and selective uptake of potential pathogens but are still poorly understood. Therefore, the carbohydrates present on crypt epithelial cells of the rabbit palatine tonsil were studied by lectin histochemistry. Ultrathin LR White sections were incubated with a panel of eight lectins conjugated to colloidal gold or biotin. The glycocalyx of the apical membrane of M-cells was selectively labeled by UEA-I, LTA, HPA, and VVA, whereas that of the remaining squamous epithelial cells preferentially bound RCA-I and PNA. WGA and ConA showed only little binding, with no discernible preference for any of the cell types. Double labeling of UEA-1 together with anti-vimentin antibodies revealed that UEA-I-positive epithelial cells also contained the rabbit M-cell marker vimentin, and vice versa. The results show that a specific composition of glycoconjugates, which differs from that on squamous epithelial cells, is found on M-cells of the rabbit tonsil. The M-cell-specific glycoproteins and glycolipids could be selectively targeted by microorganisms that adhere to M-cells and enter the host along this pathway.
Volume 44,
Issue 9,
pp. 1033-1042,
09/01/1996
Copyright © 1996 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Liu, C. K. Meagher, C. P. Moore, and T. E. Phillips M Cells in the Follicle-Associated Epithelium of the Rabbit Conjunctiva Preferentially Bind and Translocate Latex Beads Invest. Ophthalmol. Vis. Sci., November 1, 2005; 46(11): 4217 - 4223. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Carapelli, M. Regoli, C. Nicoletti, L. Ermini, L. Fonzi, and E. Bertelli Rabbit Tonsil-associated M-cells Express Cytokeratin 20 and Take Up Particulate Antigen J. Histochem. Cytochem., October 1, 2004; 52(10): 1323 - 1332. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Ouzilou, E. Caliot, I. Pelletier, M.-C. Prevost, E. Pringault, and F. Colbere-Garapin Poliovirus transcytosis through M-like cells J. Gen. Virol., September 1, 2002; 83(9): 2177 - 2182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Gebert and W. Posselt Glycoconjugate Expression Defines the Origin and Differentiation Pathway of Intestinal M-cells J. Histochem. Cytochem., October 1, 1997; 45(10): 1341 - 1350. [Abstract] [Full Text] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |