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Journal of Histochemistry and Cytochemistry, Vol. 45, 107-118, Copyright © 1997 by The Histochemical Society, Inc.

ARTICLE

In Situ Hybridization Analysis of ZPK Gene Expression During Murine Embryogenesis

André Nadeaua, Gilles Grondina, and Richard Blouina
a Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Sherbrooke, Québec, Canada

Correspondence to: Richard Blouin, Département de Biologie, Faculté des Sciences, Université de Sherbrooke, 2500 boulevard de l’Université, Sherbrooke, Québec, Canada J1K 2R1.

ZPK is a recently described protein serine/threonine kinase that has been originally identified from a human teratocarcinoma cell line by the polymerase chain reaction and whose function in signal transduction has not yet been elucidated. To investigate the potential role of this protein kinase in developmental processes, we have analyzed the spatial and temporal patterns of expression of the ZPK gene in mouse embryos of different gestational ages. Northern blot analysis revealed a single mRNA species of about 3.5 KB from Day 11 of gestation onwards. In situ hybridization studies demonstrated strong expression of ZPK mRNA in brain and in a variety of embryonic organs that rely on epithelio-mesenchymal interactions for their development, including skin, intestine, pancreas, and kidney. In these tissues, the ZPK mRNA was localized primarily in areas composed of specific types of differentiating cells, and this expression appeared to be upregulated at a time concomitant with the onset of terminal differentiation. Taken together, these observations raise the possibility that the ZPK gene product is involved in the establishment and/or maintenance of a fully cytodifferentiated state in a variety of cell lineages. (J Histochem Cy tochem 45:107-118, 1997)

Key Words: ZPK, Brain, Skin, Intestine, Pancreas, Kidney, Mouse, In situ hybridization


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