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Journal of Histochemistry and Cytochemistry, Vol. 45, 13-20, Copyright © 1997 by The Histochemical Society, Inc.

ARTICLE

Demonstration of Apoptotic Cells in Tissue Sections by In Situ Hybridization Using Digoxigenin-labeled Poly(A) Oligonucleotide Probes to Detect Thymidine-rich DNA Sequences

David A Hiltona, Seth Lovea, and Rachel Barbera
a Department of Neuropathology, Frenchay Hospital, Frenchay, Bristol, United Kingdom

Correspondence to: Seth Love, Dept. of Neuropathology, Frenchay Hospital, Bristol BS16 1LE, UK.

The recognition of apoptotic cells by morphological appearance alone may be difficult. We have investigated the use of in situ hybridization (ISH) with digoxigenin-labeled poly(A) probes to detect apoptotic cells in tissue sections. This method was compared to conventional morphologic assessment and in situ end-labeling (ISEL) in a range of tissues in which apoptosis is known to occur. ISH with poly(A) probes detected apoptotic nuclei in all tissues in which there was evidence of apoptosis as judged by conventional histology. ISH and, to a lesser extent, ISEL preferentially labeled shrunken but still intact nuclei with margination of chromatin, presumably at an early stage of apoptosis. The poly(A) hybridization was abolished by pretreatment of tissue sections with DNAse. After denaturation of DNA, poly(A) hybridized to nuclei in all cells. No convincing hybridization signal was detected in alcohol-fixed or fresh-frozen sections. Both ISEL and ISH labeled some of the nuclei in ischemic tissues. ISH with poly(A) oligonucleotide probes offers a simple alternative to ISEL for detection of cells in early stages of apoptosis. These probes hybridize to thymidine-rich sequences of DNA in the highly repeated Alu sequences within the nuclear genome. These sequences are believed to become available for hybridization after formalin fixation and paraffin embedding as a result of the apoptosis-related increase in the susceptibility of nuclear DNA to denaturation. (J Histochem Cytochem 45:13-20, 1997)

Key Words: apoptosis, in situ hybridization, poly(A), Alu sequences


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F. Labat-Moleur, C. Guillermet, P. Lorimier, C. Robert, S. Lantuejoul, E. Brambilla, and A. Negoescu
TUNEL Apoptotic Cell Detection in Tissue Sections: Critical Evaluation and Improvement
J. Histochem. Cytochem., March 1, 1998; 46(3): 327 - 334.
[Abstract] [Full Text]



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