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Journal of Histochemistry and Cytochemistry, Vol. 45, 1433-1438, Copyright © 1997 by The Histochemical Society, Inc.

ARTICLE

Differential Localization of Superoxide Dismutase Isoforms in Placental Villous Tissue of Normotensive, Pre-eclamptic, and Intrauterine Growth-restricted Pregnancies1

Leslie Myatta, Annie L.W. Eisa, Diane E. Brockmana, Wilhelm Kossenjansa, Ian A. Greerb, and Fiona Lyallb
a Departments of Obstetrics and Gynecology, Pediatrics, and Molecular and Cellular Physiology, University of Cincinnati College of Medicine, Cincinnati, Ohio
b Department of Obstetrics and Gynaecology, University of Glasgow, Glasgow, United Kingdom

Correspondence to: Leslie Myatt, Dept. of Obstetrics and Gynecology, U. of Cincinnati College of Medicine, PO Box 670526, Cincinnati, OH 45267.

Several isoforms of superoxide dismutase (SOD), including copper/zinc (cytosolic) and manganese (mitochondrial), exist. In the human placenta, SOD may prevent excessive superoxide accumulation and any potential deleterious oxidative effects. In pre-eclampsia, increased levels of lipid peroxide and decreased SOD activity have been described in the placenta. Oxidative stress such as occurs in pre-eclampsia can alter expression of SOD isoforms. The objective of this study was to localize the copper/zinc and manganese SOD isoforms in the placenta using immunohistochemistry and to compare localization and intensity of immunostaining in tissues from normotensive pregnancies with those from pregnancies complicated by pre-eclampsia and/or intrauterine growth restriction (IUGR). Western blotting with specific antibodies recognized a 17-kD copper/zinc and a 23-kD manganese SOD subunit in placental homogenates. Intense immunostaining for the manganese SOD isoform was seen in villous vascular endothelium, but only faint staining was found in the syncytiotrophoblast or villous stroma. In serial sections, intense immunostaining for copper/zinc SOD was seen in certain cells of the villous stroma but only faint immunostaining in syncytiotrophoblast and vascular endothelium. No apparent differences in localization or intensity of immunostaining for either isoform were seen between tissues of normotensive or pre-eclamptic pregnancies, with or without IUGR. The different cellular localizations of the SOD isoforms suggest that they fulfill different functional roles within the placenta.(J Histochem Cytochem 45:1433-1438, 1997)

Key Words: superoxide dismutase, placenta, pre-eclampsia, intrauterine growth restriction, superoxide, pregnancy


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