Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schmidt, B. F.
Right arrow Articles by Fisher, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schmidt, B. F.
Right arrow Articles by Fisher, G.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 45, 365-374, Copyright © 1997 by The Histochemical Society, Inc.

SYMPOSIUM ARTICLE: 47th Annual Meeting of the Histochemical Society

Signal Amplification in the Detection of Single-copy DNA and RNA by Enzyme-catalyzed Deposition (CARD) of the Novel Fluorescent Reporter Substrate Cy3.29-Tyramide

Brigitte F. Schmidta, Jean Chaoa, Zhengrong Zhua, Robin L. DeBiasioa, and Gregory Fishera
a Center for Light Microscope, Imaging and Biotechnology, Carnegie Mellon University, Pittsburgh, Pennsylvania

Correspondence to: Brigitte F. Schmidt, Center for Light Microscope, Imaging and Biotechnology, Carnegie Mellon Univ., 4400 Fifth Ave., Pittsburgh, PA 15213.

We demonstrate that the CAtalyzed Reporter Deposition method (CARD), utilizing the novel fluorescent reporter Cy3.29-tyramide, is successful in the Fluorescent In Situ Hybridization (FISH) detection of RNA and single-copy DNA. Histone 4 expression is detected in RNA extracts of S-phase, synchronized HeLa cells by dot-blot analysis. Gene expression of histone 4 in HeLa cells is demonstrated by FISH via CARD, utilizing oligonucleotide probes. Fluorescence intensity measurements on CARD-amplified histone 4 RNA detection showed (a) a 25-fold amplification of the signal brightness by biotinylated oligonucleotide probes and (b) a sixfold amplification of the signal brightness by horseradish peroxidase (HRP)-labeled histone 4 probes vs the directly stained control. The sensitivity of the CARD method is demonstrated by the FISH detection of single-copy DNA on human corneal fibroblast and HeLa S3 interphase nuclei. Chromosomal localization of the single copy DNA is demonstrated on HeLa S3 metaphase chromosome spreads. (J Histochem Cytochem 45:365-373, 1997)

Key Words: FISH (fluorescent in situ hybridization), nucleic acid detection, Cy3.29-tyramide


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Am. J. Pathol.Home page
N. Nakajima, P. Ionescu, Y. Sato, M. Hashimoto, T. Kuroita, H. Takahashi, H. Yoshikura, and T. Sata
In Situ Hybridization AT-Tailing with Catalyzed Signal Amplification for Sensitive and Specific in Situ Detection of Human Immunodeficiency Virus-1 mRNA in Formalin-Fixed and Paraffin-Embedded Tissues
Am. J. Pathol., February 1, 2003; 162(2): 381 - 389.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
A. N. Player, L.-P. Shen, D. Kenny, V. P. Antao, and J. A. Kolberg
Single-copy Gene Detection Using Branched DNA (bDNA) In Situ Hybridization
J. Histochem. Cytochem., May 1, 2001; 49(5): 603 - 612.
[Abstract] [Full Text]

Home page
 J. Histochem. Cytochem.Home page
A. U. Zaidi, H. Enomoto, J. Milbrandt, and K. A. Roth
Dual Fluorescent In Situ Hybridization and Immunohistochemical Detection with Tyramide Signal Amplification
J. Histochem. Cytochem., October 1, 2000; 48(10): 1369 - 1376.
[Abstract] [Full Text]

Home page
 J. Histochem. Cytochem.Home page
K. R. Luehrsen, S. Davidson, Y. J. Lee, R. Rouhani, A. Soleimani, T. Raich, C. A. Cain, E. J. Collarini, D. T. Yamanishi, J. Pearson, et al.
High-density Hapten Labeling and HRP Conjugation of Oligonucleotides for Use as In Situ Hybridization Probes to Detect mRNA Targets in Cells and Tissues
J. Histochem. Cytochem., January 1, 2000; 48(1): 133 - 146.
[Abstract] [Full Text]

Home page
 J. Histochem. Cytochem.Home page
H. Yang, I. B. Wanner, S. D. Roper, and N. Chaudhari
An Optimized Method for In Situ Hybridization with Signal Amplification That Allows the Detection of Rare mRNAs
J. Histochem. Cytochem., April 1, 1999; 47(4): 431 - 446.
[Abstract] [Full Text]

Home page
 J. Histochem. Cytochem.Home page
E. J.M. Speel, A. H.N. Hopman, and P. Komminoth
Amplification Methods to Increase the Sensitivity of In Situ Hybridization: Play CARD(S)
J. Histochem. Cytochem., March 1, 1999; 47(3): 281 - 288.
[Abstract] [Full Text]

Home page
 J. Histochem. Cytochem.Home page
F. Loup, O. Weinmann, Y. Yonekawa, A. Aguzzi, H.-G. Wieser, and J.-M. Fritschy
A Highly Sensitive Immunofluorescence Procedure for Analyzing the Subcellular Distribution of GABAA Receptor Subunits in the Human Brain
J. Histochem. Cytochem., October 1, 1998; 46(10): 1129 - 1140.
[Abstract] [Full Text]

Home page
 J. Histochem. Cytochem.Home page
G. Mayer and M. Bendayan
Biotinyl-Tyramide: A Novel Approach for Electron Microscopic Immunocytochemistry
J. Histochem. Cytochem., November 1, 1997; 45(11): 1449 - 1454.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
E. J.M. Speel, F. C.S. Ramaekers, and A. H.N. Hopman
Sensitive Multicolor Fluorescence In Situ Hybridization Using Catalyzed Reporter Deposition (CARD) Amplification
J. Histochem. Cytochem., October 1, 1997; 45(10): 1439 - 1446.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1997