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SYMPOSIUM ARTICLE: 47th Annual Meeting of the Histochemical Society |
Fluorochrome-labeled Tyramides: Use in Immunocytochemistry and Fluorescence In Situ Hybridization
Rob P. M. van Gijlswijka, Henry J. M. A. A. Zijlmansa, Joop Wieganta, Mark N. Bobrowb, Thomas J. Ericksonb, Karl E. Adlerb, Hans J. Tankea, and Anton K. Raapaa Department of Cytochemistry and Cytometry, Sylvius Laboratory, Leiden University, Leiden, The Netherlands
b NEN Life Science Products, Boston, Massachusetts
Correspondence to: Rob P. M. van Gijlswijk, Dept. of Cytochemistry and Cytometry, Sylvius Laboratory, Leiden Univ., Wassenaarseweg 72, 2333 AL Leiden, The Netherlands.
The peroxidase-mediated deposition of hapten- and fluorochrome-labeled tyramides has recently been shown to increase the sensitivity of immunofluoresence and fluorescence in situ hybridization techniques. We have evaluated a number of red, green, and blue fluorescent tyramides for detection of antigens in tissue sections and cytospin preparations and for the detection of hapten- and horseradish peroxidase-labeled probes hybridized in situ to cells and chromosomes. With few exceptions, all fluorescent tyramide-based methods provided a considerable increase in sensitivity compared to conventional immunofluorescence and FISH methods. (J Histochem Cytochem 45:375-382, 1997)
Key Words: TSA, fluorescence, tyramide, immunohistochemistry, in situ hybridization, peroxidase, antibodies, oligonucleotide
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