Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

This Article
Right arrow Full Text
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by D'Silva, N. J.
Right arrow Articles by Watson, E. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by D'Silva, N. J.
Right arrow Articles by Watson, E. L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 45, 965-974, Copyright © 1997 by The Histochemical Society, Inc.

ARTICLE

Immunolocalization of Rap1 in the Rat Parotid Gland: Detection on Secretory Granule Membranes

Nisha J. D'Silvaa, Dennis H. DiJulioa, Carol M. Beltona, Kerry L. Jacobsona, and E. L. Watsona,b
a Department of Oral Biology, University of Washington, Seattle, Washington
b Department of Pharmacology, University of Washington, Seattle, Washington

Correspondence to: Nisha J. D'Silva, Dept. of Oral Biology, Box 357132, U. of Washington, Seattle, WA 98195.

The objective of this study was to localize rap1 in the rat parotid gland. Rap1 is a small GTP-binding protein that has been linked to phagocytosis in neutrophils and various functions in platelets. In this study, we used [{alpha}-32P]-GTP-blot overlay analysis, immunoblot analysis, and immunohistochemistry to identify rap1 in rat parotid gland. The immunohistochemical techniques included immunoperoxidase and widefield microscopy with image deconvolution. Rap1 was identified in the secretory granule membrane (SGM), plasma membrane (PM), and cytosolic (CY) fractions, with the largest signal being in the SGM fraction. The tightly bound vs loosely adherent nature of SGM-associated rap1 was determined using sodium carbonate, and its orientation on whole granules was assessed by trypsin digestion. Rap1 was found to be a tightly bound protein rather than a loosely adherent contaminant protein of the SGM. Its orientation on the cytosolic face of the secretory granule (SG) is of significance in postulating a function for rap1 because exocytosis involves the fusion of the cytoplasmic face of the SG with the cytoplasmic face of the PM, with subsequent release of granule contents (CO). Therefore, the localization and high concentration of rap1 on the SGM and its cytosolic orientation suggest that it may play a role in the regulation of secretion. (J Histochem Cytochem 45:965-973, 1997)

Key Words: rap1, small GTP-binding protein, rat parotid gland, widefield microscopy with image deconvolution


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. A. Williams, X. Chen, and M. E. Sabbatini
Small G proteins as key regulators of pancreatic digestive enzyme secretion
Am J Physiol Endocrinol Metab, March 1, 2009; 296(3): E405 - E414.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. M. Cifuni, D. D. Wagner, and W. Bergmeier
CalDAG-GEFI and protein kinase C represent alternative pathways leading to activation of integrin {alpha}IIb{beta}3 in platelets
Blood, September 1, 2008; 112(5): 1696 - 1703.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Li, S. Asuri, J. F. Rebhun, A. F. Castro, N. C. Paranavitana, and L. A. Quilliam
The RAP1 Guanine Nucleotide Exchange Factor Epac2 Couples Cyclic AMP and Ras Signals at the Plasma Membrane
J. Biol. Chem., February 3, 2006; 281(5): 2506 - 2514.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. Farina, M. Hattori, J. Qin, Y. Nakatani, N. Minato, and K. Ozato
Bromodomain Protein Brd4 Binds to GTPase-Activating SPA-1, Modulating Its Activity and Subcellular Localization
Mol. Cell. Biol., October 15, 2004; 24(20): 9059 - 9069.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
W. T. Arthur, L. A. Quilliam, and J. A. Cooper
Rap1 promotes cell spreading by localizing Rac guanine nucleotide exchange factors
J. Cell Biol., October 11, 2004; 167(1): 111 - 122.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S Gasman, S Chasserot-Golaz, M. Popoff, D Aunis, and M. Bader
Involvement of Rho GTPases in calcium-regulated exocytosis from adrenal chromaffin cells
J. Cell Sci., January 12, 1999; 112(24): 4763 - 4771.
[Abstract] [PDF]

Home page
 CROBMHome page
E.L. Watson
GTP-Binding Proteins and Regulated Exocytosis
Critical Reviews in Oral Biology & Medicine, January 1, 1999; 10(3): 284 - 306.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
N. J. D'Silva, K. L. Jacobson, S. M. Ott, and E. L. Watson
beta -Adrenergic-induced cytosolic redistribution of Rap1 in rat parotid acini: role in secretion
Am J Physiol Cell Physiol, June 1, 1998; 274(6): C1667 - C1673.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. J. Kemppainen and E. N. Behrend
Dexamethasone Rapidly Induces a Novel Ras Superfamily Member-related Gene in AtT-20 Cells
J. Biol. Chem., February 6, 1998; 273(6): 3129 - 3131.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1997

 
Purchase HCS Short Course Manual on HCS site