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Journal of Histochemistry and Cytochemistry, Vol. 45, 1109-1120, Copyright © 1997 by The Histochemical Society, Inc.

ARTICLE

Expression of Human Group II Phospholipase A2 in Transgenic Mice

Timo J. Nevalainena, V. Jukka O. Lainea, and David S. Grassb
a Department of Pathology, University of Turku, Turku, Finland
b Chrysalis DNX Transgenic Sciences, Princeton, New Jersey

Correspondence to: Timo J. Nevalainen, Dept. of Pathology, Univ. of Turku, Kiinamyllynkatu 10, FIN-20520 Turku, Finland.

Group II phospholipase A2 (PLA2) has been proposed to play an important role in inflammation and defense against bacterial infection. We investigated tissues of transgenic mice expressing the human group II PLA2 gene by immunohistochemistry using rabbit anti-human group II PLA2 antibodies, and by in situ hybridization by probing with human group II PLA2 mRNA anti-sense (test) and sense (control) riboprobes. By immunohistochemistry, human group lI PLA2 was found in various mouse tissues and cell types including hepatocytes, proximal tubule cells of the kidney, epithelial cells of the renal pelvis, urinary bladder and ureter, granulosa cells of Graafian follicles, aortic intima and media, cartilage, epiphyseal bone, bronchial epithelial cells, and connective tissue cells in the dermis. By in situ hybridization, group ll PLA2 mRNA was localized in hepatocytes, epidermal cells, dermal cells, connective tissue fibroblasts, epithelial and smooth muscle cells of the urinary bladder, and cells of Bowman's capsule. These results show that human group ll PLA2 is expressed in large amounts in hepatocytes and many extrahepatic tissues of the transgenic mice. These animals provide a useful new tool for studies on the metabolism, in vivo effects, and physiological and pathological roles of phospholipase A2. (J Histochem Cytochem 45:1109-1119, 1997)

Key Words: acute-phase response, immunohistochemistry, inflammation, in situ hybridization, mRNA, phospholipase A2, riboprobes, transgenic mice


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