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Journal of Histochemistry and Cytochemistry, Vol. 45, 1129-1136, Copyright © 1997 by The Histochemical Society, Inc.

ARTICLE

Cytochemical Localization of Ouabain-sensitive, K+-dependent p-nitrophenylphosphatase Activity in the Facial Nerve of Reserpinized Guinea Pigs

Naoyuki Kanoha
a Department of Otolaryngology, Hyogo College of Medicine, Hyogo, Japan

Correspondence to: Naoyuki Kanoh, Dept. of Otolaryngology, Hyogo College of Medicine, 1-1 Mukogawacho, Nishinomiya, Hyogo 663, Japan.

Ion-transporting Na,K-ATPase plays an essential role in nerve conduction. To clarify the cytochemical effects of reserpine on transport Na,K-ATPase activity, the localization of ouabain-sensitive, K+-dependent p-nitrophenylphosphatase (K-NPPase) activity was investigated in the facial nerves of normal and reserpinized guinea pigs using a cerium-based method. In the normal facial nerve, the reaction product of K-NPPase activity was observed on the internodal axolemma and Schmidt-Lanterman incisures. In the Ranvier nodes, enzyme activity was localized to the paranodal and nodal axolemma. In the reserpinized nerves, reaction product was detectable on the nodal axolemma but was undetectable on the other parts of the axolemma. Nodal K-NPPase was not affected by reserpine treatment. Therefore, the transport Na,K-ATPase on the nodal axolemma might differ from that on the other parts of the axolemma. Allowing reserpinized animals to survive. Two different ouabain-sensitive K-NPPase reactivities, "reserpine-sensitive" and "reserpine-resistant," might be present in the facial nerve of guinea pigs. (J Histochem Cytochem 45:1129-1135, 1997)

Key Words: Na,K-ATPase, reserpine-sensitive, reserpine-resistant, K-NPPase cytochemistry, saltatory conduction, Ranvier node, facial nerve, guinea pig


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C.-F. Dai and N. Kanoh
Cytochemical Localization of Ouabain-sensitive, K+-dependent p-Nitrophenylphosphatase Activity in the Choroid Plexus of Normal and Reserpinized Guinea Pigs
J. Histochem. Cytochem., August 1, 1998; 46(8): 975 - 976.
[Abstract] [Full Text]



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