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Journal of Histochemistry and Cytochemistry, Vol. 45, 1239-1246, Copyright © 1997 by The Histochemical Society, Inc.

ARTICLE

Cellular Distribution of the Angiogenic Factor Heparin Affin Regulatory Peptide (HARP) mRNA and Protein in the Human Mammary Gland

Dominique Ledouxa,b, Daniele Caruellea, Jean-Christophe Sabourinc, Jianfeng Liua,b, Michel Crepinb, Denis Barritaulta, and Jose Courtya
a Laboratoire de Recherche sur la Croissance Cellulaire, la Réparation et la Régénération Tissulaires, Unité CNRS Associée 1813, Université Paris XII, Créteil, France
b Laboratoire d'Oncologie Moléculaire et Cellulaire, Université Paris XIII, Bobigny, France
c Département d'Anatomie Pathologique, Institut Gustave-Roussy, Villejuif, France

Correspondence to: Denis Barritault, Laboratoire de Recherche sur la Croissance Cellulaire, la Réparation et la Régénération Tissulaires, Unité CNRS Associée 1813, Université Paris XII, Ave. du Général de Gaulle, 94010 Créteil Cedex, France.

The heparin affin regulatory peptide (HARP) growth factor, also known as pleiotrophin, is a developmentally regulated protein that displays biological functions during cell growth and differentiation. To study the physiological role of this protein, we investigated the cellular distribution of HARP mRNA and protein in the resting human mammary gland. In situ hybridization histochemistry revealed that HARP mRNA was localized in alveolar myoepithelial cells, whereas alveolar epithelial cells were negative. In the stroma, HARP mRNA was localized in endothelial cells and smooth muscle cells of blood vessels. Interestingly, HARP protein and mRNA were not always co-localized. HARP protein immunocytochemistry staining was observed in an area including both alveolar myoepithelial and epithelial cells, although epithelial cells do not express HARP transcript. In contrast, the distribution of HARP protein is parallel to that of HARP mRNA in endothelial and vascular smooth muscle cells. In the light of these results, the putative role of HARP in controlling the proliferation and/or differentiation of the different mammary cell types is proposed and discussed. (J Histochem Cytochem 45:1239-1245, 1997)

Key Words: HARP, pleiotrophin, breast, myoepithelial cell, endothelial cell, in situ hybridization immunocytochemistry


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