Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Short, B. G.
Right arrow Articles by Schwartz, L. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Short, B. G.
Right arrow Articles by Schwartz, L. W.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 45, 1299-1306, Copyright © 1997 by The Histochemical Society, Inc.

TECHNICAL NOTE

Automated Double Labeling of Proliferation and Apoptosis in Glutathione S-transferase-positive Hepatocytes in Rats

Brian G. Shorta, Dawn M. Zimmermana, and Lester W. Schwartza
a Department of Toxicology US, SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania

Correspondence to: Brian G. Short, Senior Pathologist, SmithKline Beecham Pharm., 709 Swedeland Rd., UE0364, King of Prussia, PA 19406-0939.

Immunohistochemical markers for proliferation (bromodeoxyuridine, BrdU) and apoptosis (in situ terminal deoxynucleotide transferase dUTP nick end-labeling, TUNEL) were localized within glutathione S-transferase (GSTP)-positive hepatic foci in rats. Using the TechMate Automated Staining System (BioTek Solutions; Santa Barbara, CA), formalin-fixed, paraffin-embedded sections were run through a double-label avidin-biotin-immunoperoxidase protocol in less than 10 hr. Steam heat-induced epitope retrieval and/or proteolytic digestion preceded each labeling procedure. Color development was achieved using diaminobenzidine (DAB) with nickel enhancement for BrdU and TUNEL and VIP for GSTP. Results illustrate clear staining, brown-black BrdU-positive nuclei or TUNEL-positive apo-ptotic bodies within purple GSTP-positive hepatocytes. This automated procedure provides a method to easily identify and quantitate proliferating or apoptotic cells within foci of altered hepatocytes in rat liver and may have general applications for studies of cell or tissue kinetics during development, differentiation, and various pathological conditions in animals and humans. (J Histochem Cytochem 45:1299-1305, 1997)

Key Words: automation, proliferation, apoptosis, bromodeoxyuridine, TUNEL, steam heat, glutathione S-transferase, foci, rat


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Toxicol PatholHome page
T. Makino, K. Ishikawa, I. Igarashi, T. Yamoto, S. Manabe, and H. Nakayama
Relationship between GST Yp Induction and Hepatocyte Proliferation in Rats Treated with Phase II Drug Metabolizing Enzyme Inducers
Toxicol Pathol, April 1, 2008; 36(3): 420 - 427.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
A. Shamma, H. Yamamoto, Y. Doki, J. Okami, M. Kondo, Y. Fujiwara, M. Yano, M. Inoue, N. Matsuura, H. Shiozaki, et al.
Up-Regulation of Cyclooxygenase-2 in Squamous Carcinogenesis of the Esophagus
Clin. Cancer Res., April 1, 2000; 6(4): 1229 - 1238.
[Abstract] [Full Text]

Home page
 Clin. Cancer Res.Home page
M. Kondo, H. Yamamoto, H. Nagano, J. Okami, Y. Ito, J. Shimizu, H. Eguchi, A. Miyamoto, K. Dono, K. Umeshita, et al.
Increased Expression of COX-2 in Nontumor Liver Tissue Is Associated with Shorter Disease-free Survival in Patients with Hepatocellular Carcinoma
Clin. Cancer Res., December 1, 1999; 5(12): 4005 - 4012.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
J. Okami, H. Yamamoto, Y. Fujiwara, M. Tsujie, M. Kondo, S. Noura, S. Oshima, H. Nagano, K. Dono, K. Umeshita, et al.
Overexpression of Cyclooxygenase-2 in Carcinoma of the Pancreas
Clin. Cancer Res., August 1, 1999; 5(8): 2018 - 2024.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
G. Goping, K. A. Wood, Y. Sei, and H. B. Pollard
Detection of Fragmented DNA in Apoptotic Cells Embedded in LR White: A Combined Histochemical (LM) and Ultrastructural (EM) Study
J. Histochem. Cytochem., April 1, 1999; 47(4): 561 - 568.
[Abstract] [Full Text]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1997