ARTICLE

Distribution of H Type 1 and of H Type 2 Antigens of ABO Blood Group in Different Cells of Human Submandibular Gland

Correspondence to: Hiroshi Kimura, Dept. of Forensic Medicine, Kurume U. School of Medicine, Kurume, Fukuoka 830, Japan.

We have examined the immunohistochemical distribution of H Type 1 and of H Type 2 substances of the ABO blood group system in human submandibular gland using either of the two anti-H monoclonal antibodies MAb 1E3 and MAb 3A5. MAb 3A5 was specific for H Type 2, and MAb 1E3 reacted with each of H Type 1-H Type 4 artificial antigens. We have developed a competitive inhibition method against H Type 2 and have obtained MAb 1E3, which is fairly specific for H Type 1 under certain conditions. Mucous cells from secretors were strongly stained by 1E3 and weakly by 3A5, whereas those from nonsecretors showed no reaction with 1E3 and 3A5. Serous cells from both secretors and nonsecretors were stained neither by 1E3 nor by 3A5. Striated and interlobular duct cells were strongly stained by 1E3 and by 3A5, regardless of the secretor status. These results indicated that the expressions of the H Type 1 and H Type 2 in different cell types of the submandibular gland were controlled by different genes. In addition, we have determined the acceptor specificity of two (1,2)fucosyltransferases (H and Se enzymes) after transient expressions of the FUT1 and FUT2 in COS7 cells, and found that the H enzyme activity was similar for both Type 1 and Type 2 precursors, and that Se enzyme activity with the Type 1 precursor was higher than that with the Type 2 precursor. Expression of the H Type 1 antigen in mucous cells was found to be dependent on the Se gene, whereas expressions of the H Type 1 and H Type 2 antigens in striated and interlobular duct cells were dependent on the H gene. (J Histochem Cytochem 46:69-76, 1998)

Key Words: ABO, (1,2)fucosyltransferase, anti-H antibody, FUT1, FUT2, H antigens, immunohistochemistry, submandibular gland, UEA-I

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				A. Coddens, M. Diswall, J. Angstrom, M. E. Breimer, B. Goddeeris, E. Cox, and S. Teneberg Recognition of Blood Group ABH Type 1 Determinants by the FedF Adhesin of F18-fimbriated Escherichia coli J. Biol. Chem., April 10, 2009; 284(15): 9713 - 9726. [Abstract] [Full Text] [PDF]

				J. Holgersson and J. Lofling Glycosyltransferases involved in type 1 chain and Lewis antigen biosynthesis exhibit glycan and core chain specificity Glycobiology, July 1, 2006; 16(7): 584 - 593. [Abstract] [Full Text] [PDF]

				J. C. Lofling, E. Hauzenberger, and J. Holgersson Absorption of anti-blood group A antibodies on P-selectin glycoprotein ligand-1/immunoglobulin chimeras carrying blood group A determinants: core saccharide chain specificity of the Se and H gene encoded {alpha}1,2 fucosyltransferases in different host cells Glycobiology, March 1, 2002; 12(3): 173 - 182. [Abstract] [Full Text] [PDF]

				N. Fujitani, Y. Liu, T. Okamura, and H. Kimura Distribution of H Type 1-4 Chains of the ABO(H) System in Different Cell Types of Human Respiratory Epithelium J. Histochem. Cytochem., December 1, 2000; 48(12): 1649 - 1656. [Abstract] [Full Text]

				Y. Liu, N. Fujitani, Y. Koda, M. Soejima, and H. Kimura Presence of H Type 3/4 Chains of ABO Histo-blood Group System in Serous Cells of Human Submandibular Gland and Regulation of Their Expression by the Secretor Gene (FUT2) J. Histochem. Cytochem., July 1, 1999; 47(7): 889 - 894. [Abstract] [Full Text]

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1998