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Journal of Histochemistry and Cytochemistry, Vol. 46, 1321-1328, November 1998, Copyright © 1998, The Histochemical Society, Inc.

ARTICLE

Immunoelectron Microscopic Study for Polyamines

Kunio Fujiwaraa, Gang Baia, Tsunehiro Kitagawaa, and Daisuke Tsurub
a Faculty of Pharmaceutical Sciences, Nagasaki University, Nagasaki, Japan
b Department of Applied Microbial Technology, Kumamoto Institute of Technology, Kumamoto, Japan

Correspondence to: Kunio Fujiwara, Faculty of Pharmaceutical Sciences, Nagasaki University, Bunkyo-machi 1-14, Nagasaki 852-8131, Japan..

The polyamines (PAs) are ubiquitous polycationic metabolites in eukaryotic and prokaryotic cells and are believed to be intimately involved in the regulation of DNA, RNA, and protein biosynthesis, the exact function of which remains unclear, mainly because of a lack of knowledge of PA subcellular localization. In this study, using immunoelectron microscopy, we have demonstrated that PAs are predominantly located on free and attached ribosomes of the rough endoplasmic reticulum in the neurons of the lateral reticular nucleus of rat medulla oblongata. The nuclei, axons, and nerve endings were devoid of PA. This suggests that PAs are one of the components of biologically active ribosomes, being closely involved in the translation processes of protein biosynthesis. (J Histochem Cytochem 46:1321–1328, 1998)

Key Words: polyamines, spermine, spermidine, immunoelectron microscopy, monoclonal antibody, neuron, ribosome, protein biosynthesis, ribosomal subunit association


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