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Journal of Histochemistry and Cytochemistry, Vol. 46, 1341-1346, December 1998, Copyright © 1998, The Histochemical Society, Inc.

RAPID COMMUNICATION

A Novel Method for Concurrent Visualization of Immunostain Under Light and Electron Microscopy in Pancreatic Islets

F. Susan Wonga, Irene Visintina, Marya Shanabroughb, Csaba Leranthb, and Charles A. Janeway, Jr.a,c
a Section of Immunobiology, Yale University School of Medicine, New Haven, Connecticut
b Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, Connecticut
c Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut

Correspondence to: F. Susan Wong, Section of Immunobiology, Yale U. School of Medicine, 310 Cedar Street, New Haven, CT 06520..

We developed a simple method employing the use of flat-embedding techniques on thick frozen sections which allows correlation of light and electron microscopic immunohistochemistry. This method has been particularly useful in visualization of pancreas sections, an adaptation especially important because this tissue is not amenable to conventional vibratome sectioning. In this study we demonstrate the use of this technique to examine the same tissue section at the light and the electron microscopic level while maintaining morphology. (J Histochem Cytochem 46:1341–1345, 1998)

Key Words: immunohistochemistry, frozen section, correlative light and electron microscopy, CD8 T-cells, pancreas


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