Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Warren, K. C.
Right arrow Articles by Cary, S. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Warren, K. C.
Right arrow Articles by Cary, S. C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 46, 149-156, Copyright © 1998 by The Histochemical Society, Inc.

ARTICLE

Use of Methacrylate De-embedding Protocols for In Situ Hybridization on Semithin Plastic Sections with Multiple Detection Strategies

Kelley C. Warrena, Kathryn J. Coynea, J. Herbert Waitea, and S. Craig Carya
a Graduate College of Marine Studies, University of Delaware, Lewes, Delaware

Correspondence to: S. Craig Cary, Graduate Coll. of Marine Studies, 700 Pilottown Rd, Lewes, DE 19958.

New plastic resins are gradually replacing traditional paraffin-embedding in situ hybridization (ISH) strategies. One unique resin that has not been fully investigated or exploited with respect to light microscopic ISH is a methacrylate mixture. The advantage of this resin is its ability to be removed from tissues postsectioning, dramatically increasing hybridization signal compared to that obtained in other plastics. The goal of this study was to evaluate the general applicability of the methacrylate embedding acetone de-embedding (MEADE) technique for ISH investigations. Several high-resolution, high-sensitivity ISH protocols are described, using both end-labeled oligonucleotides and randomly primed DNA probes (200—400 BPs), signal amplification by catalyzed reporter deposition (CARD), and chromogenic and fluorescent detection methods. With slight modification, the MEADE ISH technique permitted localization of bacterial symbionts in fragile gill tissue and collagen transcripts in foot tissue of two marine bivalves. MEADE ISH has proved extremely versatile and will likely be suitable for many future applications. (J Histochem Cytochem 46:149—155, 1998)

Key Words: ISH (in situ hybridization), plastic resins, MEADE (methacrylate, embedding acetone, de-embedding), CARD (catalyzed reporter, deposition)


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Histochem. Cytochem.Home page
J. S. Torgersen, H. Takle, and O. Andersen
Localization of mRNAs and Proteins in Methyl Methacrylate-embedded Tissues
J. Histochem. Cytochem., September 1, 2009; 57(9): 825 - 830.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Zhao, C. Sun, R. J. Stewart, and J. H. Waite
Cement Proteins of the Tube-building Polychaete Phragmatopoma californica
J. Biol. Chem., December 30, 2005; 280(52): 42938 - 42944.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C. W. Tsai and S. M. Wolniak
Cell cycle arrest allows centrin translation but not basal body formation during spermiogenesis in Marsilea
J. Cell Sci., January 12, 2001; 114(23): 4265 - 4272.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
M. Mueller, K. Wacker, W. F. Hickey, E. B. Ringelstein, and R. Kiefer
Co-Localization of Multiple Antigens and Specific DNA : A Novel Method Using Methyl Methacrylate-Embedded Semithin Serial Sections and Catalyzed Reporter Deposition
Am. J. Pathol., December 1, 2000; 157(6): 1829 - 1838.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
A. R. Sipe, A. E. Wilbur, and S. C. Cary
Bacterial Symbiont Transmission in the Wood-Boring Shipworm Bankia setacea (Bivalvia: Teredinidae)
Appl. Envir. Microbiol., April 1, 2000; 66(4): 1685 - 1691.
[Abstract] [Full Text]

Home page
 J. Exp. Biol.Home page
K. Coyne and J. Waite
In search of molecular dovetails in mussel byssus: from the threads to the stem
J. Exp. Biol., January 5, 2000; 203(9): 1425 - 1431.
[Abstract] [PDF]

Home page
 J. Histochem. Cytochem.Home page
J. C. Alers, P.-J. Krijtenburg, K.\nJ. Vissers, and H. van\nDekken
Effect of Bone\nDecalcification Procedures on DNA In Situ Hybridization and\nComparative Genomic Hybridization: EDTA Is Highly Preferable to a\nRoutinely Used Acid Decalcifier
J. Histochem. Cytochem., May 1, 1999; 47(5): 703 - 710.
[Abstract] [Full Text]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1998

 
Purchase HCS Short Course Manual on HCS site