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Journal of Histochemistry and Cytochemistry, Vol. 46, 231-240, Copyright © 1998 by The Histochemical Society, Inc.

ARTICLE

Intestinal Carbamoyl Phosphate Synthase I in Human and Rat: Expression During Development Shows Species Differences and Mosaic Expression in Duodenum of Both Species

Erik H. Van Beersa, Edmond H.H.M. Ringsa, George Posthumab, Maria A. Dingemansec, Jan A.M.J. Taminiaua, Hugo S.A. Heymansa, Alexandra W.C. Einerhanda, Hans A. Büllerd, and Jan Dekkera
a Pediatric Gastroenterology and Nutrition, Department Pediatrics, Emma Children's Hospital, Academic Medical Center, Amsterdam, The Netherlands
b Department of Cell Biology, School of Medicine, University of Utrecht, The Netherlands
c Department of Anatomy and Embryology, Academic Medical Center, University of Amsterdam, The Netherlands
d Sophia Children's Hospital, Erasmus University Rotterdam, The Netherlands

Correspondence to: Jan Dekker, Pediatric Gastroenterology and Nutrition, Dept. of Pediatrics, Academic Medical Center, Rm G8-205, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands.

The clinical importance of carbamoyl phosphate synthase I (CPSI) relates to its capacity to metabolize ammonia, because CPSI deficiencies cause lethal serum ammonia levels. Although some metabolic parameters concerning liver and intestinal CPSI have been reported, the extent to which enterocytes contribute to ammonia conversion remains unclear without a detailed description of its developmental and spatial expression patterns. Therefore, we determined the patterns of enterocytic CPSI mRNA and protein expression in human and rat intestine during embryonic and postnatal development, using in situ hybridization and immunohistochemistry. CPSI protein appeared during human embryogenesis in liver at 31—35 e.d. (embryonic days) before intestine (59 e.d.), whereas in rat CPSI detection in intestine (at 16 e.d.) preceded liver (20 e.d.). During all stages of development there was a good correlation between the expression of CPSI protein and mRNA in the intestinal epithelium. Strikingly, duodenal enterocytes in both species exhibited mosaic CPSI protein expression despite uniform CPSI mRNA expression in the epithelium and the presence of functional mitochondria in all epithelial cells. Unlike rat, CPSI in human embryos was expressed in liver before intestine. Although CPSI was primarily regulated at the transcriptional level, CPSI protein appeared mosaic in the duodenum of both species, possibly due to post-transcriptional regulation. (J Histochem Cytochem 46:231—240, 1998)

Key Words: rat, human, biopsy, embryogenesis, development, carbamoyl phosphate, synthase I, intestine, enterocyte, in situ hybridization, immunohistochemistry


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