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Journal of Histochemistry and Cytochemistry, Vol. 46, 301-312, Copyright © 1998, The Histochemical Society, Inc.

ARTICLE

Angiotensinogen, Prorenin, and Renin Are Co-localized in the Secretory Granules of All Glandular Cells of the Rat Anterior Pituitary: An Immunoultrastructural Study

Evelyne Vila–Porcilea and Pierre Corvola
a Collège de France, INSERM U 36, Paris, France

Correspondence to: Evelyne Vila–Porcile, Biologie de la Cellule Neuroendocrine, Collège de France, 11 Place Marcelin Berthelot, 75231 Paris Cedex 05, France.

In addition to the circulating renin–angiotensin system (RAS), a local system has been postulated in the anterior pituitary because immunodetection of its components in various mammalian species. However, different cell types appear to be involved in different species, and there is no general consensus on the subcellular localization of prorenin, renin and angiotensinogen. In this ultrastructural study, we investigated and quantified the presence of these components using double or triple immunogold labeling methods, in all the immunologically identified glandular cell types of the rat anterior pituitary. In contrast to previous reports, all these components were identified not only in lactotropes and gonadotropes but also in somatotropes, corticotropes, and thyrotropes. The highest levels were detected in lactotropes and gonadotropes, and renin gave the greatest signal. Angiotensinogen, prorenin, and renin were co-localized in the secretory granules of all rat pituitary glandular cell types. The simultaneous detection of the substrate (angiotensinogen) and both its specific cleavage enzyme and its proenzyme within the same granule suggests intragranular processing of this component. Moreover, the localization of these three constituents in the secretory granules also suggests that, in the rat anterior pituitary, they follow the regulated secretory pathway. (J Histochem Cytochem 46:301–311, 1998)

Key Words: angiotensinogen, prorenin, renin, anterior pituitary cells, immunogold, electron microscopy, rat (Wistar)


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