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ARTICLE |
Laminin-5 Expression Is Independent of the Injury and the Microenvironment During Reepithelialization of Wounds
Tiina Kainulainena, Lari Häkkinene, Sara Hamidie, Kirsi Larjavae, Matti Kallioinenb, Juha Peltonenc, Tuula Saloa, Hannu Larjavae, and Aarne Oikarinenda Department of Oral and Maxillofacial Surgery, University of Oulu, Oulu, Finland
b Department of Pathology, University of Oulu, Oulu, Finland
c Department of Anatomy, University of Oulu, Oulu, Finland
d Department of Dermatology, University of Oulu, Oulu, Finland
e Department of Clinical Dental Sciences, University of British Columbia, Vancouver, BC, Canada
Correspondence to: Aarne Oikarinen, Dept. of Dermatology, Univ. of Oulu, Fin-90220 Oulu, Finland.
We examined the expression of laminin-5 and its integrin receptors during reepithelialization of human wounds. We used suction blisters of skin as a model of keratinocyte migration on a basement membrane matrix and mucosal full-thickness wounds as a model in which keratinocytes migrate in a provisional matrix. An animal model, in which human epidermal keratinocytes were injected into the back of athymic mice, was used to follow the deposition of the basement membrane components. In 4-day-old blisters, about 20–50 cells at the leading edge of the migrating tongue showed cytoplasmic laminin-5 immunostaining. Laminin-5 mRNA was detected in 15–30 cells at the leading edge of the migrating epidermis. 
3ß1 and 6ß4 integrins were found in membrane projections of the migrating basal cells and also in suprabasal cell layers, suggesting their combined role in binding laminin-5. In mucosal wounds, laminin-5 was the only basement membrane zone component that was deposited between the clot and the migrating keratinocytes. In the animal model, linear deposition of laminin-5 and 6ß4 integrin was already seen on Day 2, whereas the other basement membrane zone components were not yet organized. The results suggest that, regardless of the injury and the microenvironment, laminin-5 plays an essential role in the interaction between wound keratinocytes and the surrounding matrix. (J Histochem Cytochem 46:353–360, 1998)
Key Words: wound healing, suction blister, anchoring filament, integrins, cytokines, in situ hybridization
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