Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gerdes, M. J.
Right arrow Articles by Rowley, D. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gerdes, M. J.
Right arrow Articles by Rowley, D. R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 46, 379-388, Copyright © 1998, The Histochemical Society, Inc.

ARTICLE

Localization of Transforming Growth Factor-b1 and Type II Receptor in Developing Normal Human Prostate and Carcinoma Tissues

Michael J. Gerdesa, Melinda Larsenb, Lauren McBridea, Truong D. Danga, Bing Lua, and David R. Rowleya,b
a Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030
b Cell and Molecular Biology Program, Baylor College of Medicine, Houston, Texas 77030

Correspondence to: David R. Rowley, Dept. of Cell Biology, 466A, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030.

Transforming growth factor-ß1 (TGF-ß1) is implicated in prostate development, and elevated expression of TGF-ß1 has been correlated with prostate carcinogenesis. In this study, cell type specificity of TGF-ß1 and TGF-ß receptor Type II (RcII) protein expression was determined by immunocytochemistry in human normal prostate and compared to prostate carcinoma tissues. Heterogeneous localization patterns of LAP-TGF-ß1 (TGF-ß1 precursor) and RcII were observed in both epithelial and mesenchymal cells in fetal prostate, with LAP-TGF-ß1 localizing to more basal epithelial cells. Homogeneity of LAP-TGF-ß1 staining was increased in neonatal, prepubertal, and adult prostate, with elevated immunoreactivity noted in epithelial acini relative to stromal tissue for both LAP-TGF-ß1 and RcII proteins. In stromal tissues, RcII cell localization exhibited staining patterns nearly identical to smooth muscle {alpha}-actin. In prostate carcinoma, LAP-TGF-ß1 localized to carcinoma cells with an increased staining heterogeneity relative to normal prostate. In contrast to normal epithelial cells, carcinoma epithelial cells exhibited low to nondetectable RcII staining. Stromal cell staining patterns for LAP-TGF-ß1 and RcII in carcinoma, however, were identical to those of normal prostate stromal cells. These studies implicate both epithelial and stromal cells as sites of TGF-ß1 synthesis and RcII localization in the developing and adult normal human prostate. In addition, these data indicate a loss of epithelial expression of RcII concurrent with altered LAP-TGF-ß1 expression in human prostate carcinoma cells. (J Histochem Cytochem 46:379–388, 1998)

Key Words: TGF-ß1, TGF-ß receptor type II, prostate, human, cancer, development


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 aacredbookHome page
B. A Teicher, J. M Yingling, and J. M McPherson
TGF{beta} Blockade as Anticancer Therapy
Am. Assoc. Cancer Res. Educ. Book, April 12, 2008; 2008(1): 71 - 81.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
B. A. Teicher
Transforming Growth Factor-{beta} and the Immune Response to Malignant Disease
Clin. Cancer Res., November 1, 2007; 13(21): 6247 - 6251.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Epidemiol. Biomarkers Prev.Home page
T. M. Beer, A. Myrthue, M. Garzotto, M. F. O'Hara, R. Chin, B. A. Lowe, M. A. Montalto, C. L. Corless, and W. D. Henner
Randomized Study of High-Dose Pulse Calcitriol or Placebo prior to Radical Prostatectomy
Cancer Epidemiol. Biomarkers Prev., December 1, 2004; 13(12): 2225 - 2232.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
D. C. Tomlinson, S. H. Freestone, O. C. Grace, and A. A. Thomson
Differential Effects of Transforming Growth Factor-{beta}1 on Cellular Proliferation in the Developing Prostate
Endocrinology, September 1, 2004; 145(9): 4292 - 4300.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
I. V. Litvinov, A. M. De Marzo, and J. T. Isaacs
Is the Achilles' Heel for Prostate Cancer Therapy a Gain of Function in Androgen Receptor Signaling?
J. Clin. Endocrinol. Metab., July 1, 2003; 88(7): 2972 - 2982.
[Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
J. A. Tuxhorn, G. E. Ayala, M. J. Smith, V. C. Smith, T. D. Dang, and D. R. Rowley
Reactive Stroma in Human Prostate Cancer: Induction of Myofibroblast Phenotype and Extracellular Matrix Remodeling
Clin. Cancer Res., September 1, 2002; 8(9): 2912 - 2923.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. E. Bassi, R. L. De Cicco, H. Mahloogi, S. Zucker, G. Thomas, and A. J. P. Klein-Szanto
Furin inhibition results in absent or decreased invasiveness and tumorigenicity of human cancer cells
PNAS, August 17, 2001; (2001) 191199198.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. J. Gerdes, T. D. Dang, M. Larsen, and D. R. Rowley
Transforming Growth Factor-{beta}1 Induces Nuclear to Cytoplasmic Distribution of Androgen Receptor and Inhibits Androgen Response in Prostate Smooth Muscle Cells
Endocrinology, August 1, 1998; 139(8): 3569 - 3577.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. E. Bassi, R. Lopez De Cicco, H. Mahloogi, S. Zucker, G. Thomas, and A. J. P. Klein-Szanto
Furin inhibition results in absent or decreased invasiveness and tumorigenicity of human cancer cells
PNAS, August 28, 2001; 98(18): 10326 - 10331.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1998