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Journal of Histochemistry and Cytochemistry, Vol. 46, 487-496, April 1998, Copyright © 1998, The Histochemical Society, Inc.

ARTICLE

Herpes simplex Virus Type 1-infected Human Embryonic Lung Cells Studied by Optimized Immunogold Cryosection Electron Microscopy

Helle L. Jensena and Bodil Norrilda
a The Protein Laboratory, Institute of Molecular Pathology, The DNA Tumour Virus Group, University of Copenhagen, The Panum Institute, Copenhagen, Denmark

Correspondence to: Helle L. Jensen, the Protein Laboratory, the DNA Tumour Virus Group, University of Copenhagen, The Panum Institute, Bldg. 6.2., Blegdamsvej 3C, DK-2200 Copenhagen N, Denmark.

Herpes simplex virus type 1 (HSV-1) is a common human pathogen of skin and mucous membranes and is potentially dangerous when the infection is disseminated. Viral morphogenesis, especially the mechanism of viral envelopment and the exact pathway for processing and transport of HSV-1 glycoproteins, is still unclear. We report the results of optimized immunogold-labeled cryosection electron microscopy of HSV-1-infected cultured human fibroblasts (MRC-5). The simplified method presented has proved necessary to obtain reproducible results on cellular distribution of viral glycoproteins. It is now possible to demonstrate the viral glycoprotein gD-1, but not gC-1, in the nuclear membranes and to demonstrate gD-1- and gC-1-labeled viral particles in the perinuclear space, and to show the fate of the viral particles in the endoplasmic reticulum and Golgi area in infected cells. (J Histochem Cytochem 46:487–496, 1998)

Key Words: cryosection, electron microscopy, glycoproteins, HSV-1, immunogold


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