Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bréchot, J.-M.
Right arrow Articles by Bernaudin, J.-F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bréchot, J.-M.
Right arrow Articles by Bernaudin, J.-F.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 46, 513-518, April 1998, Copyright © 1998, The Histochemical Society, Inc.

ARTICLE

Different Pattern of MRP Localization in Ciliated and Basal Cells from Human Bronchial Epithelium

Jeanne-Marie Bréchota, Ilse Hurbaina, Anne Fajaca, Nicole Datya, and Jean-François Bernaudina
a Laboratoire d'Histologie–Biologie Tumorale, Université Pierre et Marie Curie Paris VI, Hôpital Tenon, Paris, France

Correspondence to: Jeanne-Marie Bréchot, Hôtel-Dieu, 1 Place du Parvis Notre-Dame, 75181 Paris Cedex 04, France.

The MRP (multidrug resistance-associated protein) transmembrane transporter, which actively transports a wide variety of lipophilic substrates out of cancer cells, has been suggested to play a major role in cell detoxification via efflux of glutathione conjugates. Because bronchial epithelial cells are constantly exposed to environmental pollutants, MRP might be a particularly important defense mechanism against xenobiotics. This study was therefore designed to investigate MRP localization by immunohistochemistry in bronchial epithelial cells collected by scraping from surgical specimens. In parallel, MRP mRNA was detected by reverse transcriptase chain reaction (rt-PCR) in bronchial cell lysates. However, the pattern of protein expression differed markedly according to cell type. In ciliated epithelial cells, immunostaining was restricted to the basolateral surface, without any labeling at the apical surface, which is at variance with the localization of CFTR and MDR1 proteins, other members of the same family of transporters. In basal cells, MRP was present over the entire circumference of the plasma membrane. Basal cells were identified by their morphology and specifically after incubation with an anticytokeratin 17 monoclonal antibody. In conclusion, the different patterns of localization suggest specific roles for MRP in basal and ciliated cells. (J Histochem Cytochem 46:513–517, 1998)

Key Words: MRP, bronchial epithelium, basal cells, ciliated cells


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Histochem. Cytochem.Home page
L. Bouhamyia, S. Chantot-Bastaraud, S. Zaidi, P. Roynard, C. Prengel, J.-F. Bernaudin, and J. Fleury-Feith
Immunolocalization and Cell Expression of Lung Resistance-related Protein (LRP) in Normal and Tumoral Human Respiratory Cells
J. Histochem. Cytochem., August 1, 2007; 55(8): 773 - 782.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
G. Horvath, N. Schmid, M. A. Fragoso, A. Schmid, G. E. Conner, M. Salathe, and A. Wanner
Epithelial Organic Cation Transporters Ensure pH-Dependent Drug Absorption in the Airway
Am. J. Respir. Cell Mol. Biol., January 1, 2007; 36(1): 53 - 60.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
R. G. Deeley, C. Westlake, and S. P. C. Cole
Transmembrane Transport of Endo- and Xenobiotics by Mammalian ATP-Binding Cassette Multidrug Resistance Proteins.
Physiol Rev, July 1, 2006; 86(3): 849 - 899.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
J. J. Lima, S. Zhang, A. Grant, L. Shao, K. G. Tantisira, H. Allayee, J. Wang, J. Sylvester, J. Holbrook, R. Wise, et al.
Influence of Leukotriene Pathway Polymorphisms on Response to Montelukast in Asthma
Am. J. Respir. Crit. Care Med., February 15, 2006; 173(4): 379 - 385.
[Abstract] [Full Text] [PDF]

Home page
 Drug Metab. Dispos.Home page
A. Guo, W. Marinaro, P. Hu, and P. J. Sinko
Delineating the Contribution of Secretory Transporters in the Efflux of Etoposide Using Madin-Darby Canine Kidney (MDCK) Cells Overexpressing P-Glycoprotein (Pgp), Multidrug Resistance-Associated Protein (MRP1), and Canalicular Multispecific Organic Anion Transporter (cMOAT)
Drug Metab. Dispos., April 1, 2002; 30(4): 457 - 463.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
K. O. Hamilton, E. Topp, I. Makagiansar, T. Siahaan, M. Yazdanian, and K. L. Audus
Multidrug Resistance-Associated Protein-1 Functional Activity in Calu-3 Cells
J. Pharmacol. Exp. Ther., September 1, 2001; 298(3): 1199 - 1205.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
M.-A. Wioland, J. Fleury–Feith, P. Corlieu, F. Commo, G. Monceaux, J. Lacau-St-Guily, and J.-F. Bernaudin
CFTR, MDR1, and MRP1 Immunolocalization in Normal Human Nasal Respiratory Mucosa
J. Histochem. Cytochem., September 1, 2000; 48(9): 1215 - 1222.
[Abstract] [Full Text]

Home page
 J. Histochem. Cytochem.Home page
K.-C. Peng, F. Cluzeaud, M. Bens, J.-P. D. Van Huyen, M. A. Wioland, R. Lacave, and A. Vandewalle
Tissue and Cell Distribution of the Multidrug Resistance-Associated Protein (MRP) in Mouse Intestine and Kidney
J. Histochem. Cytochem., June 1, 1999; 47(6): 757 - 768.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
K.-i. Ito, C. J. Oleschuk, C. Westlake, M. Z. Vasa, R. G. Deeley, and S. P. C. Cole
Mutation of Trp1254 in the Multispecific Organic Anion Transporter, Multidrug Resistance Protein 2 (MRP2) (ABCC2), Alters Substrate Specificity and Results in Loss of Methotrexate Transport Activity
J. Biol. Chem., October 5, 2001; 276(41): 38108 - 38114.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1998