Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Unger, E. R.
Right arrow Articles by Reeves, W. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Unger, E. R.
Right arrow Articles by Reeves, W. C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 46, 535-540, April 1998, Copyright © 1998, The Histochemical Society, Inc.

TECHNICAL NOTE

Detection of Human Papillomavirus in Archival Tissues: Comparison of In Situ Hybridization and Polymerase Chain Reaction

Elizabeth R. Ungera, Suzanne D. Vernonb, Daisy R. Leea, Donna L. Millerb, and William C. Reevesb
a Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia
b Division of Viral and Rickettsial Disease, Center for Infectious Disease, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia

Correspondence to: Elizabeth R. Unger, Centers for Disease Control and Prevention, 1600 Clifton Road, MSG18, Atlanta, GA 30333, eru0{at}cdc.gov (E-mail).

Formalin-fixed, paraffin-embedded tissues in pathology archives are an important resource for molecular epidemiology studies. Use of these tissues requires that assays be optimized to account for inevitable variations in tissue fixation and processing that occur in the performance of routine histology. We compared results of colorimetric in situ hybridization (ISH) to L1 consensus polymerase chain reaction (PCR) for detection and typing of human papillomavirus (HPV) in 180 blocks of archival tissues (up to 9 years in storage) from cervical cancer patients. Fifteen samples could not be amplified by PCR, but assays were concordant in 75.1% (124/165) of samples that could be analyzed by both methods. Similar numbers of ISH+/PCR- (23) and ISH-/PCR+ (18) cases were found. Eight of the 18 ISH-/PCR+ cases were attributable to PCR detection of HPV types not included in the ISH assay. This degree of concordance required individual optimization of assay conditions for each block. ISH and PCR assays for HPV yield complementary results, and both can be successfully applied to archival tissues. (J Histochem Cytochem 46:535–540, 1998)

Key Words: colorimetric in situ, hybridization, human papillomavirus, polymerase chain reaction, archival tissues, formalin fixation


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Clin. Microbiol.Home page
M. Guo, Y. Gong, M. Deavers, E. G. Silva, Y. J. Jan, D. E. Cogdell, R. Luthra, E. Lin, H. C. Lai, W. Zhang, et al.
Evaluation of a Commercialized In Situ Hybridization Assay for Detecting Human Papillomavirus DNA in Tissue Specimens from Patients with Cervical Intraepithelial Neoplasia and Cervical Carcinoma
J. Clin. Microbiol., January 1, 2008; 46(1): 274 - 280.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
A. Tsezou, P. Oikonomou, P. Kollia, I. Mademtzis, E. Kostopoulou, I. Messinis, and N. Vamvakopoulos
The Role of Human Telomerase Catalytic Subunit mRNA Expression in Cervical Dysplasias
Experimental Biology and Medicine, April 1, 2005; 230(4): 263 - 270.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1998