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Journal of Histochemistry and Cytochemistry, Vol. 46, 541-546, April 1998, Copyright © 1998, The Histochemical Society, Inc.

TECHNICAL NOTE

A New Method for Intense Staining of Myelin

Karen J. McNallya and Alan Petersa
a Department of Anatomy and Neurobiology, Boston University School of Medicine, Boston, Massachusetts

Correspondence to: Alan Peters, Dept. of Anatomy and Neurobiology, Boston U. School of Medicine, 80 East Concord St., Boston, MA 02118.

We describe a new method for intense staining of myelin. The stain involves immersing frozen or vibratome sections in 4% normal horse serum. A DAB reaction is then carried out, which results in the deposition of reaction product in myelin sheaths. On intensification of this reaction product using the silver enhancement technique described by Görcs, myelin stains an intense black color, making the preparations suitable for photography. The stain is especially useful for determining the distribution of myelinated fibers in gray matter. (J Histochem Cytochem 46:541–545, 1998)

Key Words: myelin, horse serum, DAB reaction, silver intensification


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