Vascular Permeability Factor/Vascular Endothelial Growth Factor and Vascular Stroma Formation in Neoplasia: Insights from In Situ Hybridization StudiesLawrence F. Browna, Anthony J. Guidia, Kathi Tognazzia, and Harold F. Dvorakaa Departments of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts Correspondence to: Lawrence F. Brown, Dept. of Pathology, Beth Israel Deaconess Medical Center, East Campus, 330 Brookline Ave., Boston, MA 02215. The formation of vascular stroma plays an important role in the pathophysiology of malignancy. We describe the use of in situ hybridization in our laboratory as a tool to study the role of vascular permeability factor/vascular endothelial growth factor in the angiogenesis associated with malignancy. (J Histochem Cytochem 46:569575, 1998) Key Words: VPF, VEGF, angiogenesis, KDR, flt-1, carcinoma
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