Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Øster, A.
Right arrow Articles by Larsson, L.-I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Øster, A.
Right arrow Articles by Larsson, L.-I.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 46, 707-716, June 1998, Copyright © 1998, The Histochemical Society, Inc.

ARTICLE

Rat Endocrine Pancreatic Development in Relation to Two Homeobox Gene Products (Pdx-1 and Nkx 6.1)

Anne Østera, Jan Jensenb, Palle Serupb, Philip Galanteb, Ole D. Madsenb, and Lars-Inge Larssona
a Department of Molecular Cell Biology, Statens Seruminstitut, Copenhagen
b Hagedorn Research Institute, Gentofte, Denmark

Correspondence to: Lars-Inge Larsson, Dept. of Molecular Cell Biology, Statens Seruminstitut, Artillerivej 5, Bldg. 81, DK-2300 Copenhagen S, Denmark.

We studied the distribution of the homeodomain proteins Pdx-1 and Nkx 6.1 in the developing rat pancreas. During early development, nuclear staining for both Pdx-1 and Nkx 6.1 occurred in most epithelial cells of the pancreatic anlage. Subsequently, Nkx 6.1 became more ß-cell-restricted, and Pdx-1 also occurred in other islet cell types and in the duodenal epithelium. During early pancreatic development, cells co-storing insulin and glucagon were regularly detected. The vast majority of these did not possess nuclear staining for either Pdx-1 or Nkx 6.1. Subsequently, cells storing insulin only appeared. Such cells displayed strongly Pdx-1- and Nkx 6.1-positive nuclei. Therefore, Nkx 6.1, like Pdx-1, may be an important factor in pancreatic development and in mature insulin cell function.

(J Histochem Cytochem 46:707–715, 1998)

Key Words: homeodomain proteins, Nkx 6.1, Pdx-1, insulin, pancreatic polypeptide, glucagon


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Histochem. Cytochem.Home page
M. Kodama, F. Takeshita, S. Kanegasaki, T. Ochiya, and G. Quinn
Pancreatic Endocrine and Exocrine Cell Ontogeny From Renal Capsule transplanted Embryonic Stem Cells in Streptozocin-injured Mice
J. Histochem. Cytochem., January 1, 2008; 56(1): 33 - 44.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
M. C. Jorgensen, J. Ahnfelt-Ronne, J. Hald, O. D. Madsen, P. Serup, and J. Hecksher-Sorensen
An Illustrated Review of Early Pancreas Development in the Mouse
Endocr. Rev., October 1, 2007; 28(6): 685 - 705.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
F. C. Lynn, S. B. Smith, M. E. Wilson, K. Y. Yang, N. Nekrep, and M. S. German
Sox9 coordinates a transcriptional network in pancreatic progenitor cells
PNAS, June 19, 2007; 104(25): 10500 - 10505.
[Abstract] [Full Text] [PDF]

Home page
 Eur J EndocrinolHome page
M. E Cerf
Transcription factors regulating {beta}-cell function.
Eur. J. Endocrinol., November 1, 2006; 155(5): 671 - 679.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
I. Rooman, N. De Medts, L. Baeyens, J. Lardon, S. De Breuck, H. Heimberg, and L. Bouwens
Expression of the Notch Signaling Pathway and Effect on Exocrine Cell Proliferation in Adult Rat Pancreas
Am. J. Pathol., October 1, 2006; 169(4): 1206 - 1214.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. C. Raum, K. Gerrish, I. Artner, E. Henderson, M. Guo, L. Sussel, J. C. Schisler, C. B. Newgard, and R. Stein
FoxA2, Nkx2.2, and PDX-1 Regulate Islet {beta}-Cell-Specific mafA Expression through Conserved Sequences Located between Base Pairs -8118 and -7750 Upstream from the Transcription Start Site
Mol. Cell. Biol., August 1, 2006; 26(15): 5735 - 5743.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
I. L. Pedersen, R. Klinck, J. Hecksher-Sorensen, S. Zahn, O. D. Madsen, P. Serup, and M. C. Jorgensen
Generation and Characterization of Monoclonal Antibodies against the Transcription Factor Nkx6.1
J. Histochem. Cytochem., May 1, 2006; 54(5): 567 - 574.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
M. E. Wilson, K. Y. Yang, A. Kalousova, J. Lau, Y. Kosaka, F. C. Lynn, J. Wang, C. Mrejen, V. Episkopou, H. C. Clevers, et al.
The HMG Box Transcription Factor Sox4 Contributes to the Development of the Endocrine Pancreas
Diabetes, December 1, 2005; 54(12): 3402 - 3409.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
K. D. Henseleit, S. B. Nelson, K. Kuhlbrodt, J. C. Hennings, J. Ericson, and M. Sander
NKX6 transcription factor activity is required for {alpha}- and {beta}-cell development in the pancreas
Development, July 1, 2005; 132(13): 3139 - 3149.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Schonhoff, L. Baggio, C. Ratineau, S. K. Ray, J. Lindner, M. A. Magnuson, D. J. Drucker, and A. B. Leiter
Energy Homeostasis and Gastrointestinal Endocrine Differentiation Do Not Require the Anorectic Hormone Peptide YY
Mol. Cell. Biol., May 15, 2005; 25(10): 4189 - 4199.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Zhao, M. Guo, T.-a. Matsuoka, D. K. Hagman, S. D. Parazzoli, V. Poitout, and R. Stein
The Islet {beta} Cell-enriched MafA Activator Is a Key Regulator of Insulin Gene Transcription
J. Biol. Chem., March 25, 2005; 280(12): 11887 - 11894.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. L. Prado, A. E. Pugh-Bernard, L. Elghazi, B. Sosa-Pineda, and L. Sussel
Ghrelin cells replace insulin-producing {beta} cells in two mouse models of pancreas development
PNAS, March 2, 2004; 101(9): 2924 - 2929.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T.-a. Matsuoka, I. Artner, E. Henderson, A. Means, M. Sander, and R. Stein
The MafA transcription factor appears to be responsible for tissue-specific expression of insulin
PNAS, March 2, 2004; 101(9): 2930 - 2933.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
N. Wierup, S. Yang, R. J. McEvilly, H. Mulder, and F. Sundler
Ghrelin Is Expressed in a Novel Endocrine Cell Type in Developing Rat Islets and Inhibits Insulin Secretion from INS-1 (832/13) Cells
J. Histochem. Cytochem., March 1, 2004; 52(3): 301 - 310.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
P. Collombat, A. Mansouri, J. Hecksher-Sorensen, P. Serup, J. Krull, G. Gradwohl, and P. Gruss
Opposing actions of Arx and Pax4 in endocrine pancreas development
Genes & Dev., October 15, 2003; 17(20): 2591 - 2603.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Vincent, Y. Guz, M. Rozenberg, G. Webb, M. Furuta, D. Steiner, and G. Teitelman
Abrogation of Protein Convertase 2 Activity Results in Delayed Islet Cell Differentiation and Maturation, Increased {alpha}-Cell Proliferation, and Islet Neogenesis
Endocrinology, September 1, 2003; 144(9): 4061 - 4069.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
B. W. Kahan, L. M. Jacobson, D. A. Hullett, J. M. Ochoada, T. D. Oberley, K. M. Lang, and J. S. Odorico
Pancreatic Precursors and Differentiated Islet Cell Types From Murine Embryonic Stem Cells: An In Vitro Model to Study Islet Differentiation
Diabetes, August 1, 2003; 52(8): 2016 - 2024.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
J. Buteau, S. Foisy, E. Joly, and M. Prentki
Glucagon-Like Peptide 1 Induces Pancreatic {beta}-Cell Proliferation Via Transactivation of the Epidermal Growth Factor Receptor
Diabetes, January 1, 2003; 52(1): 124 - 132.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
J. Movassat, G. M. Beattie, A. D. Lopez, and A. Hayek
Exendin 4 Up-Regulates Expression of PDX 1 and Hastens Differentiation and Maturation of Human Fetal Pancreatic Cells
J. Clin. Endocrinol. Metab., October 1, 2002; 87(10): 4775 - 4781.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
D. R. Laybutt, G. C. Weir, H. Kaneto, J. Lebet, R. D. Palmiter, A. Sharma, and S. Bonner-Weir
Overexpression of c-Myc in {beta}-Cells of Transgenic Mice Causes Proliferation and Apoptosis, Downregulation of Insulin Gene Expression, and Diabetes
Diabetes, June 1, 2002; 51(6): 1793 - 1804.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
J. Buteau, S. Foisy, C. J. Rhodes, L. Carpenter, T. J. Biden, and M. Prentki
Protein Kinase C{zeta} Activation Mediates Glucagon-Like Peptide-1-Induced Pancreatic {beta}-Cell Proliferation
Diabetes, October 1, 2001; 50(10): 2237 - 2243.
[Abstract] [Full Text]

Home page
 J. Histochem. Cytochem.Home page
C. Roberts, G. A. Roberts, K. Löbner, M. Bearzatto, A. Clark, E. Bonifacio, and M. R. Christie
Expression of the Protein Tyrosine Phosphatase-like Protein IA-2 During Pancreatic Islet Development
J. Histochem. Cytochem., June 1, 2001; 49(6): 767 - 776.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
P. Jacquemin, S. M. Durviaux, J. Jensen, C. Godfraind, G. Gradwohl, F. Guillemot, O. D. Madsen, P. Carmeliet, M. Dewerchin, D. Collen, et al.
Transcription Factor Hepatocyte Nuclear Factor 6 Regulates Pancreatic Endocrine Cell Differentiation and Controls Expression of the Proendocrine Gene ngn3
Mol. Cell. Biol., June 15, 2000; 20(12): 4445 - 4454.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
R. G. Mirmira, H. Watada, and M. S. German
beta -Cell Differentiation Factor Nkx6.1 Contains Distinct DNA Binding Interference and Transcriptional Repression Domains
J. Biol. Chem., May 5, 2000; 275(19): 14743 - 14751.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
G. Gradwohl, A. Dierich, M. LeMeur, and F. Guillemot
neurogenin3 is required for the development of the four endocrine cell lineages of the pancreas
PNAS, February 15, 2000; 97(4): 1607 - 1611.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
M Sander, L Sussel, J Conners, D Scheel, J Kalamaras, F Dela Cruz, V Schwitzgebel, A Hayes-Jordan, and M German
Homeobox gene Nkx6.1 lies downstream of Nkx2.2 in the major pathway of beta-cell formation in the pancreas
Development, January 12, 2000; 127(24): 5533 - 5540.
[Abstract] [PDF]

Home page
 DevelopmentHome page
V. Schwitzgebel, D. Scheel, J. Conners, J Kalamaras, J. Lee, D. Anderson, L Sussel, J. Johnson, and M. German
Expression of neurogenin3 reveals an islet cell precursor population in the pancreas
Development, January 8, 2000; 127(16): 3533 - 3542.
[Abstract] [PDF]

Home page
 DevelopmentHome page
M Gannon, M. Ray, K Van Zee, F Rausa, R. Costa, and C. Wright
Persistent expression of HNF6 in islet endocrine cells causes disrupted islet architecture and loss of beta cell function
Development, January 7, 2000; 127(13): 2883 - 2895.
[Abstract] [PDF]

Home page
 J. Histochem. Cytochem.Home page
A. Øster, J. Jensen, H. Edlund, and L.-I. Larsson
Homeobox Gene Product Nkx 6.1 Immunoreactivity in Nuclei of Endocrine Cells of Rat and Mouse Stomach
J. Histochem. Cytochem., June 1, 1998; 46(6): 717 - 722.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
H. Watada, R. G. Mirmira, J. Leung, and M. S. German
Transcriptional and Translational Regulation of beta -Cell Differentiation Factor Nkx6.1
J. Biol. Chem., October 27, 2000; 275(44): 34224 - 34230.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. E. Allen-Jennings, M. G. Hartman, G. J. Kociba, and T. Hai
The Roles of ATF3 in Glucose Homeostasis. A TRANSGENIC MOUSE MODEL WITH LIVER DYSFUNCTION AND DEFECTS IN ENDOCRINE PANCREAS
J. Biol. Chem., July 27, 2001; 276(31): 29507 - 29514.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1998

 
Purchase HCS Short Course Manual on HCS site