Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Calkins, C. C.
Right arrow Articles by Moin, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Calkins, C. C.
Right arrow Articles by Moin, K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 46, 745-752, June 1998, Copyright © 1998, The Histochemical Society, Inc.

ARTICLE

Differential Localization of Cysteine Protease Inhibitors and a Target Cysteine Protease, Cathepsin B, by Immuno–Confocal Microscopy

Cathárine C. Calkinsa, Mansoureh Samenia, Jennifer Koblinskia, Bonnie F. Sloanea, and Kamiar Moina
a Department of Pharmacology, Wayne State University, Detroit, Michigan

Correspondence to: Kamiar Moin, Dept. of Pharmacology, Wayne State U. School of Medicine, 540 E. Canfield, Detroit, MI 48201.

The cystatin superfamily of cysteine protease inhibitors and target cysteine proteases such as cathepsin B have been implicated in malignant progression. The respective cellular/extracellular localization of cystatins and cysteine proteases in tumors may be critical in regulating activity of the enzymes. Confocal microscopy has enabled us to demonstrate the differential localization of cystatins and cathepsin B in an embryonic liver cell line and an invasive hepatoma cell line. In both, stefins A and B were distributed diffusely throughout the cytoplasm, whereas cystatin C was distributed in juxtanuclear vesicles. Stefin A and cystatin C, but not stefin B, were present on the cell surface. Cystatin C was found on the top surfaces of both cell lines, whereas stefin A was found only on the top surface of the embryonic liver cells. Cathepsin B staining was concentrated in perinuclear vesicles in the embryonic liver cells. In the hepatoma cells, staining for cathepsin B was also present in vesicles adjacent to the cell membrane and on localized regions of the bottom surface. Such a disparate distribution of cathepsin B and its endogenous inhibitors may facilitate proteolysis by the hepatoma cells and thereby contribute to their invasive phenotype. (J Histochem Cytochem 46:745–751, 1998)

Key Words: cathepsin, cystatin, cysteine protease inhibitor, immunocytochemistry, stefin


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 JCOHome page
R. D. Petty, K. M. Kerr, G. I. Murray, M. C. Nicolson, P. H. Rooney, D. Bissett, and E. S.R. Collie-Duguid
Tumor Transcriptome Reveals the Predictive and Prognostic Impact of Lysosomal Protease Inhibitors in Non-Small-Cell Lung Cancer
J. Clin. Oncol., April 10, 2006; 24(11): 1729 - 1744.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
W. Li, F. Ding, L. Zhang, Z. Liu, Y. Wu, A. Luo, M. Wu, M. Wang, Q. Zhan, and Z. Liu
Overexpression of Stefin A in Human Esophageal Squamous Cell Carcinoma Cells Inhibits Tumor Cell Growth, Angiogenesis, Invasion, and Metastasis
Clin. Cancer Res., December 15, 2005; 11(24): 8753 - 8762.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
J. P. Sokol and W. P. Schiemann
Cystatin C Antagonizes Transforming Growth Factor {beta} Signaling in Normal and Cancer Cells
Mol. Cancer Res., March 1, 2004; 2(3): 183 - 195.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1998