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Journal of Histochemistry and Cytochemistry, Vol. 46, 767-770, June 1998, Copyright © 1998, The Histochemical Society, Inc.

TECHNICAL NOTE

A Histochemical Approach to Correlative Light and Electron Microscopic Detection of Acidic Glycoconjugates by a Sensitized High Iron Diamine Method

Yoshifumi Hirabayashia and Kazuyori Yamadaa
a Department of Anatomy, Nagoya City University Medical School, Nagoya, Japan

Correspondence to: Yoshifumi Hirabayashi, Dept. of Anatomy, Nagoya City Univ. Medical School, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467, Japan.

A sensitized high iron diamine method is among the reliable and useful histochemical means of detecting acidic glycoconjugates by light microscopy. Because the final reaction products obtained using this method are heavy metals, it can be applied to specimens for visualization by both light and electron microscopy. In this study the high iron diamine method was utilized successfully as a correlative light and electron microscopic method for detection of acidic glycoconjugates. (J Histochem Cytochem 46:767–770, 1998)

Key Words: histochemistry, correlative light and electron, microscopy, acidic glycoconjugates, sensitized high iron diamine


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