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Journal of Histochemistry and Cytochemistry, Vol. 46, 779-782, June 1998, Copyright © 1998, The Histochemical Society, Inc.

TECHNICAL NOTE

Staining of the Midbody by an Anti-digoxin-specific Antibody

Roeland W. Dirksa and Anton K. Raapa
a Department of Molecular Cell Biology, Laboratory of Cytochemistry and Cytometry, Leiden University, Leiden, The Netherlands

Correspondence to: Roeland W. Dirks, Dept. of Molecular Cell Biology, Lab. of Cytochemistry and Cytometry, Leiden University, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands.

Using RNA in situ hybridization to reveal cytoplasmic localization patterns of mRNAs in cultured cells, we noted unexpected staining of a cytoplasmic component in telophase cells. Control experiments revealed that the anti-digoxin-specific antibody was responsible for this staining. Because the staining was observed only at a position where both daughter cells are still connected, we identified the stained component as the midbody. This was confirmed by double staining of cells with anti-digoxin and anti-{alpha}-tubulin antibodies. We concluded that anti-digoxin-specific antibody shows crossreactivity with a component present in the midbody. (J Histochem Cytochem 46:779–782, 1998)

Key Words: digoxin, midbody, crossreactivity, RNA in situ hybridization


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